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UNITED STATES
SECURITIES AND EXCHANGE COMMISSION
Washington, D.C. 20549
FORM
N-CSR
CERTIFIED SHAREHOLDER REPORT OF REGISTERED
MANAGEMENT INVESTMENT COMPANIES
Investment Company Act file number:
811-23983
Gladstone Alternative Income Fund
(Exact name of registrant as specified in charter)
1521 Westbranch Drive, Suite 100
McLean, Virginia 22102
(Address of principal executive offices) (Zip code)
(703)
287-5800
(Registrant’s telephone number, including area code)
David Gladstone
Gladstone Alternative Income Fund
1521 Westbranch Drive, Suite 100
McLean, Virginia 22102
(Name and address of agent for service)
Date of fiscal year end: March 31
Date of reporting period: March 31, 2026

Item 1. Reports to Stockholders.

(a) |

Table of Contents

Shareholder Letter (Unaudited) |  |  | 3 |
Schedule of Investments |  |  | 6 |
Statement of Assets and Liabilities |  |  | 8 |
Statement of Operations |  |  | 9 |
Statements of Changes in Net Assets |  |  | 10 |
Statement of Cash Flows |  |  | 11 |
Financial Highlights |  |  | 13 |
Notes to Financial Statements |  |  | 15 |
Report of Independent Registered Public Accounting Firm |  |  | 32 |
Additional Information (Unaudited) |  |  | 33 |
Trustees & Officers (Unaudited) |  |  | 36 |
Investor Data Privacy Notice (Unaudited) |  |  | 39 |

Gladstone Alternative Income Fund | Shareholder Letter

March 31, 2026 (Unaudited)
Dear Shareholder,
This annual report of the Gladstone Alternative Income Fund (the “Fund”) covers the period from the beginning of our fiscal year, April 1, 2025, through March 31, 2026. Since commencing operations in December 2024, we have worked to create a portfolio consistent with our Fund’s investment strategy of primarily investing in directly originated loans to lower and middle market private businesses in the United States, broadly syndicated loans and commercial real estate loans. Consistent with our investment strategy we have also made equity investments, in connection with our directly originated loans. As of March 31, 2026, the Fund was invested in seven issuers across seven industries. The holdings in the Fund’s portfolio include investments in a number of asset categories including secured debt, preferred and common equity. 100% of the Fund’s portfolio (excluding investments in U.S. Treasury obligations, short-term investments and reverse repurchase agreements) consists of
co-investments
with one of the business development companies also advised by Gladstone Management Corporation, either Gladstone Investment Corporation or Gladstone Capital Corporation, highlighting the benefit of the Gladstone platform to the Fund.
Capital markets during the period ended March 31, 2026 continued to experience periods of volatility, driven by evolving expectations around interest rates, inflation and geopolitical developments, among other items. In light of this backdrop, we have maintained a disciplined focus on directly originated loans to lower middle market businesses, complemented by allocations to U.S. Treasury securities and cash to preserve adequate liquidity and manage risk. Our portfolio is invested in industries and issuers that we believe are resilient to economic uncertainty, including aerospace and defense, food, and other essential business and consumer services, with an emphasis on senior secured lending structures with equity
co-investments
to support the long-term investment plans. While the capital-raising environment has been slower than expected, we continue to focus on generating risk-adjusted returns for our shareholders while providing access to a broad portfolio of private lower middle market credit and equity investments.
Top 10 Investment Holdings^

Holdings |  | Percentage |
United States Treasury Bill |  |  | 30.33 | %
Morgan Stanley Institutional Liquidity Fund (MSILF) Government Portfolio |  |  | 12.46 | %
Detroit Defense (Secured First Lien Debt) |  |  | 12.34 | %
Dutch Gold Honey (Secured Second Lien Debt) |  |  | 9.10 | %
Viron International (Secured First Lien Debt) |  |  | 6.87 | %

Holdings |  | Percentage |
Clean Water Environmental Services, Inc. (Secured First Lien Debt) |  |  | 6.74 | %
Smart Chemical Solutions (Secured First Lien Debt) |  |  | 5.42 | %
Nielsen-Kellerman (Secured First Lien Debt) |  |  | 4.68 | %
Flexible Technology Solutions (Secured Second Lien Debt) |  |  | 3.28 | %
Dutch Gold Honey (Common Stock) |  |  | 2.91 | %

Annual Report | March 31, 2026 |  | 3

Gladstone Alternative Income Fund | Shareholder Letter

March 31, 2026 (Unaudited)
Allocation by Industry^

Industry |  | Percentage |
United States Treasury Bill |  |  | 30.33 | %
Aerospace & Defense |  |  | 14.87 | %
Money Markets |  |  | 12.46 | %
Food & Tobacco |  |  | 12.01 | %
Diversified/Conglomerate Manufacturing |  |  | 6.87 | %
Ecological |  |  | 6.74 | %
Business/Consumer Services |  |  | 6.52 | %
Electronics |  |  | 6.08 | %
Diversified/Conglomerate Service |  |  | 4.12 | %

Allocation by Asset Type^

Asset |  | Percentage |
Direct Lending (Floating Rate) |  |  | 45.15 | %
United States Treasury Bill |  |  | 30.33 | %
Money Markets |  |  | 12.46 | %
Equities (Private, Preferred) |  |  | 5.87 | %
Direct Lending (Fixed Rate) |  |  | 3.28 | %
Equities (Private, Common) |  |  | 2.91 | %

^ | % of Investments and Short Term Investments, at value

For the fiscal year ended March 31, 2026, the Fund returned 6.6% (including reinvested dividends), reflecting performance at the net asset value of Class A shares, compared to its benchmark, the Standard & Poor’s 500 Total Return Index, which returned 14.5% on an annualized basis over the same period. Since its inception in December 2024, the Fund returned 5.0% (including reinvested dividends), compared to 5.0% for the Standard & Poor’s 500 Total Return Index.
The following graph shows the value, as of March 31, 2026, of a hypothetical $10,000 investment made on December 9, 2024 (the Fund’s inception date) in Class A Shares at net asset value (with a sales charge of 5.75% and without such sales charge). The chart accounts for dividend reinvestment on the monthly dividends that have been paid from March 2025 through March 2026. For comparative purposes, the performance of the S&P 500 Total Return Index is shown. The Fund believes that the S&P 500 Total Return Index, which assumes reinvestment of dividends, is an appropriate broad-based securities market index for comparison purposes. Performance reflects applicable fee waivers and/or expense limitations in effect during the periods shown and in their absence, performance would be reduced. Returns do not reflect the deduction of taxes that a shareholder would pay on Fund distributions or the repurchase of Fund shares. The returns in the graph and table set forth below represent past performance. Past performance does not guarantee future results. The Fund’s investment return and principal value will fluctuate so that an investor’s shares, when repurchased, may be worth more or less than their original cost. Current performance may be lower or higher than the performance quoted below. Please visit our website at https://www.gladstoneintervalfund.com to obtain the most recent
month-end
returns.

4 |  | www.gladstoneintervalfund.com

Gladstone Alternative Income Fund | Shareholder Letter

March 31, 2026 (Unaudited)
Comparison Cumulative Return on Investment of $10,000
As we move forward into our next fiscal year and as the Fund continues to ramp its portfolio, we intend to work diligently to acquire appropriate investments for the Fund by leveraging the Gladstone platform.

1 | U.S. Treasury obligations, short-term investments and reverse repurchase agreements are not included in this discussion of the Fund’s portfolio.

Annual Report | March 31, 2026 |  | 5

Gladstone Alternative Income Fund | Schedule of Investments

March 31, 2026

Equity Investments - 12.15%(a) |  | Shares |  |  | Value |
Food & Tobacco - 4.03% |  |  |  |  |  |  |  |
Dutch Gold Honey, Inc. (Common Stock) (b)(c)(d) |  |  | 100,000 |  |  | $ | 864,069 |
Diversified/Conglomerate Service - 1.16% |  |  |  |  |  |  |  |
Flexible Technology Solutions, LLC (Preferred Stock) (b) (c)(d) |  |  | 2,500 |  |  |  | 250,000 |
Electronics - 1.94% |  |  |  |  |  |  |  |
Nielsen-Kellerman Acquisition Corp. (Preferred Stock) (b)(c)(e) |  |  | 631 |  |  |  | 416,820 |
Aerospace & Defense - 3.50% |  |  |  |  |  |  |  |
Detroit Defense, Inc. (Preferred Stock) (b)(c)(e) |  |  | 1,039 |  |  |  | 750,839 |
Business/Consumer Services - 1.52% |  |  |  |  |  |  |  |
Smart Chemical Solutions, LLC (Preferred Stock) (b)(c)(e) |  |  | 617 |  |  |  | 326,638 |
Diversified/Conglomerate Manufacturing - 0.00% |  |  |  |  |  |  |  |
Viron International Corp. (Common Stock) (b)(c)(d) |  |  | 50 |  |  |  | 0 |
Total Equity Investments (Cost $2,551,346) |  |  |  |  |  | $ | 2,608,366 |

Debt Investments - 66.95% (a) |  | Rate |  | Maturity Date |  |  | Principal Amount |  |  | Value |
Ecological - 9.32% |  |  |  |  |  |  |  |  |  |  |  |  |  |
Clean Water Environmental Services, Inc. (Secured First Lien Debt) (c)(d) |  | Term SOFR + 7.3% |  |  | 2/18/2031 |  |  | $ | 2,000,000 |  |  | $ | 2,000,000 |
Food & Tobacco - 12.58% |  |  |  |  |  |  |  |  |  |  |  |  |  |
Dutch Gold Honey, Inc. (Secured Second Lien Debt) (c)(d) |  | Term SOFR + 7.5%, 2% Floor |  |  | 8/4/2030 |  |  |  | 2,700,000 |  |  |  | 2,700,000 |
Diversified/Conglomerate Service - 4.53% |  |  |  |  |  |  |  |  |  |  |  |  |  |
Flexible Technology Solutions, LLC (Secured Second Lien Debt) (c)(d) |  | 11.5% |  |  | 3/31/2031 |  |  |  | 1,000,000 |  |  |  | 971,870 |
Electronics - 6.47% |  |  |  |  |  |  |  |  |  |  |  |  |  |
Nielsen-Kellerman Corp. (Secured First Lien Debt) (c)(e) |  | Term SOFR + 8.5%, 13.5% Floor |  |  | 12/19/2029 |  |  |  | 1,368,858 |  |  |  | 1,388,802 |
Aerospace & Defense - 17.06% |  |  |  |  |  |  |  |  |  |  |  |  |  |
Detroit Defense, Inc. (Secured First Lien Debt) (c)(e) |  | Term SOFR + 9%, 13% Floor |  |  | 12/31/2029 |  |  |  | 3,661,466 |  |  |  | 3,661,466 |
Business/Consumer Services - 7.49% |  |  |  |  |  |  |  |  |  |  |  |
Smart Chemical Solutions, LLC (Secured First Lien Debt) (c)(e) |  | Term SOFR + 9%, 13.5% Floor |  |  | 5/15/2030 |  |  |  | 1,589,823 |  |  |  | 1,608,106 |

See Notes to Financial Statements. |  |
6 |  | www.gladstoneintervalfund.com

Gladstone Alternative Income Fund | Schedule of Investments

March 31, 2026

Debt Investments - 66.95% (a) |  | Rate |  | Maturity Date |  |  | Principal Amount |  |  | Value |
Diversified/Conglomerate Manufacturing - 9.50% |  |  |  |  |  |  |  |  |  |  |  |  |  |
Viron International Corp. (Secured First Lien Debt) (c)(d) |  | Term SOFR + 7% |  |  | 2/7/2030 |  |  | $ | 2,042,500 |  |  | $ | 2,039,253 |
Total Debt Investments (Cost $14,362,645) |  |  |  |  |  |  |  |  |  | $ | 14,369,497 |
Investments, at fair value |  |  |  |  |  |  |  |  |  |  |  | $ | 16,977,863 |
Short Term Investments - 59.16% |  |  |  |  |  |  |  |  |  |  |

United States Treasury Obligations - 41.93% |  | Rate |  |  | Maturity Date |  | Principal Amount |  | Value |
United States Treasury Bill (f) |  |  | 3.46 | % |  | 4/2/2026 |  | $9,000,000 |  | $ | 8,999,099 |
Money Market Fund - 17.23% |  |  |  |  |  | 7 Day Yield |  | Shares |  |  | Value |
MSILF Government Portfolio (g) |  |  |  |  |  | 3.55% |  | 3,697,074 |  | $ | 3,697,074 |
Total Short Term Investments (Cost $12,696,221) |  |  |  |  |  |  |  |  |  | $ | 12,696,173 |
Total Investments - 138.26%; (Cost $29,610,212) |  |  |  |  |  |  |  |  |  | $ | 29,674,036 |
Liabilities in Excess of Other Assets (38.26%) |  |  |  |  |  |  |  |  |  |  | (8,211,663 | )
Net Assets - 100.00% |  |  |  |  |  |  |  |  |  | $ | 21,462,373 |

Investment Abbreviations:
SOFR - Secured Overnight Financing Rate
Reference Rates:
Term SOFR - 30 Day SOFR as of March 31, 2026 was 3.652%

(a) | All investments are in unaffiliated companies located in the United States.

(b) | Non-income producing security.

(c) | As a result of the use of significant unobservable inputs to determine fair value, these investments have been classified as Level 3 securities under the fair value hierarchy.

(d) | One of our affiliated funds, Gladstone Capital Corporation, co-invested with us in this portfolio company pursuant to an exemptive order granted by the U.S. Securities and Exchange Commission.

(e) | One of our affiliated funds, Gladstone Investment Corporation, co-invested with us in this portfolio company pursuant to an exemptive order granted by the U.S. Securities and Exchange Commission.

(f) | All or partial amount transferred for the benefit of the counterparty as collateral for reverse repurchase agreements. As of March 31, 2026, the value pledged was $8,548,269.

(g) | The rate shown is the annualized 7-day yield as of March 31, 2026. Valued using Level 1 inputs within the FASB ASC 820 fair value hierarchy. Refer to Note 3—Investments in the accompanying Notes to Financial Statements for additional information.

Reverse Repurchase Agreements

Counterparty |  | Interest Rate |  |  | Acquisition Date |  |  | Maturity Date |  |  | Amount Borrowed |  |  | Amount Payable |
UMB |  |  | 4.40 | % |  |  | 3/27/2026 |  |  |  | 04/02/2026 |  |  | $ | 8,100,000 |  |  | $ | 8,101,980 |

See Notes to Financial Statements. |  |
Annual Report | March 31, 2026 |  | 7

Gladstone Alternative Income Fund |  | Statement of Assets and Liabilities

March 31, 2026

ASSETS |  |  |  |
Investments, at fair value (Cost $16,913,991) |  | $ | 16,977,863 |
Short-term investments, at fair value (Cost $12,696,221) |  |  | 12,696,173 |
Cash |  |  | 50,522 |
Dividend and interest receivable |  |  | 153,731 |
Receivable due from Adviser (Note 7) |  |  | 154,267 |
Prepaid expenses and other assets |  |  | 67,856 |
Total assets |  |  | 30,100,412 |
LIABILITIES |  |  |  |
Distributions payable to common shareholders |  |  | 55,473 |
Administration and fund accounting fees payable |  |  | 25,331 |
Administrative reimbursement payable to Administrator (Note 7) |  |  | 320,541 |
Transfer agent fees payable |  |  | 27,595 |
Distribution and shareholder servicing fee payable (Note 7) |  |  | 2,443 |
Trustees fees and expenses payable |  |  | 20 |
Payable for reverse repurchase agreement, including accrued interest of $1,980 |  |  | 8,101,980 |
Due to Adviser (Note 7) |  |  | 30,549 |
Accrued expenses and other liabilities |  |  | 74,107 |
Total liabilities |  |  | 8,638,039 |
Commitments and contingencies (Note 2) |  |  |  |
NET ASSETS |  | $ | 21,462,373 |
COMPONENTS OF NET ASSETS |  |  |  |
Paid-in capital |  | $ | 21,444,395 |
Distributable Earnings/(Losses) |  |  | 17,978 |
NET ASSETS |  | $ | 21,462,373 |
Net Assets By Share Class |  |  |  |
Class I Shares |  | $ | 20,703,796 |
Class C Shares |  |  | 145,829 |
Class A Shares |  |  | 612,748 |
|  | $ | 21,462,373 |
Shares of Beneficial Interest Outstanding (unlimited number of shares of no par value): |  |  |  |
Class I Shares |  |  | 2,068,105 |
Class C Shares |  |  | 14,759 |
Class A Shares |  |  | 61,390 |
|  |  | 2,144,254 |
Net Asset Value per Share: |  |  |  |
Class I Shares |  | $ | 10.01 |
Class C Shares |  | $ | 9.88 |
Class A Shares |  | $ | 9.98 |

See Notes to Financial Statements. |  |
8 |  | www.gladstoneintervalfund.com

Gladstone Alternative Income Fund |  | Statement of Operations

|  | For the Year Ended March 31, 2026 |
INVESTMENT INCOME |  |  |  |
Dividend income |  | $ | 401,265 |
Interest income |  |  | 1,629,734 |
Prepayment income |  |  | 44,992 |
Total investment income |  |  | 2,075,991 |
EXPENSES |  |  |  |
Advisory fees (Note 7) |  |  | 259,508 |
Administration and fund accounting fees |  |  | 252,867 |
Administrative reimbursement to Administrator (Note 7) |  |  | 848,924 |
Transfer agent fees |  |  | 156,138 |
Distribution and shareholder servicing fee - Class A (Note 7) |  |  | 787 |
Distribution and shareholder servicing fee - Class C (Note 7) |  |  | 1,172 |
Professional fees |  |  | 591,829 |
Amortization of offering costs |  |  | 534,329 |
Custodian fees |  |  | 38,260 |
Interest expense (Note 8) |  |  | 30,812 |
Trustees’ fees and expenses |  |  | 62,482 |
Insurance fees |  |  | 123,605 |
Printing fees |  |  | 50,738 |
Other |  |  | 180,143 |
Total expenses |  |  | 3,131,594 |
Less Advisory fee credits (Notes 7) |  |  | (117,169 | )
Less expenses waived and reimbursed by Adviser (Notes 7 and 10) |  |  | (2,304,650 | )
Total Advisory fee credit waivers and expenses waived and reimbursed by Adviser (Notes 7 and 10) |  |  | (2,421,819 | )
Net expenses |  |  | 709,775 |
NET INVESTMENT INCOME |  | $ | 1,366,216 |
NET REALIZED GAIN/(LOSS) AND CHANGE IN UNREALIZED APPRECIATION/(DEPRECIATION) FROM INVESTMENTS |  |  |  |
Net change in unrealized appreciation/(depreciation) on Investments |  | $ | 58,677 |
NET REALIZED GAIN/(LOSS) AND CHANGE IN UNREALIZED APPRECIATION/(DEPRECIATION) ON INVESTMENTS |  |  | 58,677 |
NET INCREASE IN NET ASSETS RESULTING FROM OPERATIONS |  | $ | 1,424,893 |

See Notes to Financial Statements. |  |
Annual Report | March 31, 2026 |  | 9

Gladstone Alternative Income Fund |  | Statements of Changes in Net Assets

|  | For the Year Ended March 31, 2026 |  |  | For the Period December 9, 2024 (Commencement of Operations) to March 31, 2025 |
OPERATIONS |  |  |  |  |  |  |  |
Net investment income |  | $ | 1,366,216 |  |  | $ | 110,459 |
Net realized gain/(loss) from Investments |  |  | — |  |  |  | — |
Net change in unrealized appreciation/(depreciation) from Investments |  |  | 58,677 |  |  |  | 5,147 |
Net increase in net assets resulting from operations |  |  | 1,424,893 |  |  |  | 115,606 |
DISTRIBUTIONS |  |  |  |  |  |  |  |
Class I |  |  | (1,386,145 | ) |  |  | (104,873 | )
Class C |  |  | (8,099 | ) |  |  | (527 | )
Class A |  |  | (21,811 | ) |  |  | (1,233 | )
Net decrease in net assets from distributions |  |  | (1,416,055 | ) |  |  | (106,633 | )
SHARE TRANSACTIONS |  |  |  |  |  |  |  |
Class I |  |  |  |  |  |  |  |
Proceeds from shares issued |  |  | 25,000 |  |  |  | 19,800,000 |
Reinvestment of distributions |  |  | 758,327 |  |  |  | — |
Net increase from share transactions |  |  | 783,327 |  |  |  | 19,800,000 |
Class C |  |  |  |  |  |  |  |
Proceeds from shares issued |  |  | 40,000 |  |  |  | 100,000 |
Reinvestment of distributions |  |  | 7,661 |  |  |  | — |
Net increase from share transactions |  |  | 47,661 |  |  |  | 100,000 |
Class A |  |  |  |  |  |  |  |
Proceeds from shares issued |  |  | 369,402 |  |  |  | 234,000 |
Reinvestment of distributions |  |  | 10,172 |  |  |  | — |
Net increase from share transactions |  |  | 379,574 |  |  |  | 234,000 |
Net increase in net assets from share transactions |  |  | 1,210,562 |  |  |  | 20,134,000 |
NET ASSETS |  |  |  |  |  |  |  |
Beginning of period (Note 1) |  |  | 20,242,973 |  |  |  | 100,000 |
End of period |  | $ | 21,462,373 |  |  | $ | 20,242,973 |
Fund Share Transactions |  |  |  |  |  |  |  |
Class I |  |  |  |  |  |  |  |
Shares Sold |  |  | 2,468 |  |  |  | 1,980,000 |
Reinvestment of distributions |  |  | 75,637 |  |  |  | — |
Net increase in shares outstanding |  |  | 78,105 |  |  |  | 1,980,000 |
Class C |  |  |  |  |  |  |  |
Shares Sold |  |  | 3,988 |  |  |  | 10,000 |
Reinvestment of distributions |  |  | 771 |  |  |  | — |
Net increase in shares outstanding |  |  | 4,759 |  |  |  | 10,000 |
Class A |  |  |  |  |  |  |  |
Shares Sold |  |  | 36,973 |  |  |  | 23,400 |
Reinvestment of distributions |  |  | 1,017 |  |  |  | — |
Net increase in shares outstanding |  |  | 37,990 |  |  |  | 23,400 |

See Notes to Financial Statements. |  |
10 |  | www.gladstoneintervalfund.com

Gladstone Alternative Income Fund |  | Statement of Cash Flows

|  | For the Year Ended March 31, 2026 |
Cash Flows from Operating Activities: |  |  |  |
Net increase in net assets resulting from operations |  | $ | 1,424,893 |
Adjustments to reconcile net increase in net assets resulting from operations to net cash provided by operating activities: |  |  |  |
Purchase of Investments |  |  | (43,211,016 | )
Sale of Investments |  |  | 40,539,169 |
Net proceeds from short-term investments |  |  | 2,055,985 |
Net change in unrealized appreciation/(depreciation) on Investments |  |  | (58,677 | )
Discount and premiums amortized |  |  | (14,800 | )
Amortization of offering costs |  |  | 534,329 |
(Increase)/Decrease in Assets: |  |  |  |
Interest receivable |  |  | (29,100 | )
Due from Adviser |  |  | (60,048 | )
Prepaid expenses and other assets |  |  | (4,170 | )
Increase/(Decrease) in Liabilities: |  |  |  |
Administration and fund accounting fees payable |  |  | (1,266 | )
Transfer agent fees payable |  |  | (18,479 | )
Distribution and shareholder servicing fee payable |  |  | 1,959 |
Trustees fees and expenses payable |  |  | (842 | )
Administrative fee reimbursement payable to Administrator |  |  | 61,766 |
Interest due on reverse repurchase agreement |  |  | (3,154 | )
Due to Adviser |  |  | 30,549 |
Accrued expenses and other liabilities |  |  | (39,923 | )
Net cash provided by operating activities |  |  | 1,207,175 |
Cash Flows from Financing Activities: |  |  |  |
Proceeds from shares issued |  |  | 434,402 |
Cash distributions paid |  |  | (691,055 | )
Proceeds from reverse repurchase agreements |  |  | 33,300,000 |
Payments on reverse repurchase agreements |  |  | (34,200,000 | )
Net cash used in financing activities |  |  | (1,156,653 | )
Cash, beginning of year |  |  | — |
Net change in cash |  |  | 50,522 |
Cash, end of year |  | $ | 50,522 |
Non-Cash Financing Activities: |  |  |  |
Reinvestment of distributions |  | $ | 776,160 |
Supplemental Disclosure of Cash Flow Information: |  |  |  |
Cash paid for interest expense |  |  | 33,967 |

See Notes to Financial Statements. |  |
Annual Report | March 31, 2026 |  | 11

Gladstone Alternative Income Fund |  | Financial Highlights - Class I
|  | March 31, 2026

|  | For the Year Ended March 31, 2026 |  |  | For the Period December 9, 2024 (Commencement of operations) to March 31, 2025 |
NET ASSET VALUE, BEGINNING OF PERIOD |  | $ | 10.00 |  |  | $ | 10.00 |
INCOME FROM INVESTMENT OPERATIONS |  |  |  |  |  |  |  |
Net investment income (a) |  |  | 0.66 |  |  |  | 0.06 |
Net realized and unrealized gain/(loss) on investments (a) |  |  | 0.04 |  |  |  | (0.01 | )
Total income from investment operations (a) |  |  | 0.70 |  |  |  | 0.05 |
DISTRIBUTIONS |  |  |  |  |  |  |  |
From net investment income (a) |  |  | (0.69 | ) |  |  | (0.05 | )
Total distributions (a) |  |  | (0.69 | ) |  |  | (0.05 | )
NET ASSET VALUE, END OF PERIOD |  | $ | 10.01 |  |  | $ | 10.00 |
TOTAL RETURN (b) |  |  | 7.17 | % |  |  | 0.53 | %
RATIOS AND SUPPLEMENTAL DATA |  |  |  |  |  |  |  |
Net assets, end of period (000s) |  | $ | 20,704 |  |  | $ | 19,909 |
RATIOS TO AVERAGE NET ASSETS |  |  |  |  |  |  |  |
Ratio of gross expenses to average net assets |  |  | 15.06 | % (c) |  |  | 17.23 | % (c)
Ratio of expense waiver/reimbursements to average net assets |  |  | (11.66 | %) (c) |  |  | (11.49 | %) (c)
Ratio of net expenses to average net assets |  |  | 3.40 | % (c) |  |  | 5.74 | % (c)
Ratio of net investment income to average net assets |  |  | 6.59 | % (c) |  |  | 1.87 | % (c)
PORTFOLIO TURNOVER RATE |  |  | 16.00 | % |  |  | 0.00 | %

(a) | Per share numbers have been calculated using the average shares method.

(b) | Based on net asset value per share. Distributions, if any, are assumed for purposes of this calculation to be reinvested at prices obtained under the Fund’s Dividend Reinvestment Plan. Total return is not annualized for periods less than one year.

(c) | Annualized (except organizational costs and non-recurring expenses).

See Notes to Financial Statements. |  |
12 |  | www.gladstoneintervalfund.com

Gladstone Alternative Income Fund |  | Financial Highlights - Class C
|  | March 31, 2026

|  | For the Year Ended March 31, 2026 |  |  | For the Period December 9, 2024 (Commencement of operations) to March 31, 2025 |
NET ASSET VALUE, BEGINNING OF PERIOD |  | $ | 9.97 |  |  | $ | 10.00 |
INCOME FROM INVESTMENT OPERATIONS |  |  |  |  |  |  |  |
Net investment income (a) |  |  | 0.56 |  |  |  | 0.02 |
Net realized and unrealized gain/(loss) on investments (a) |  |  | 0.04 |  |  |  | — |
Total income from investment operations (a) |  |  | 0.60 |  |  |  | 0.02 |
DISTRIBUTIONS |  |  |  |  |  |  |  |
From net investment income (a) |  |  | (0.69 | ) |  |  | (0.05 | )
Total distributions (a) |  |  | (0.69 | ) |  |  | (0.05 | )
NET ASSET VALUE, END OF PERIOD |  | $ | 9.88 |  |  | $ | 9.97 |
TOTAL RETURN (b) |  |  | 6.16 | % |  |  | 0.23 | %
RATIOS AND SUPPLEMENTAL DATA |  |  |  |  |  |  |  |
Net assets, end of period (000s) |  | $ | 146 |  |  | $ | 100 |
RATIOS TO AVERAGE NET ASSETS |  |  |  |  |  |  |  |
Ratio of gross expenses to average net assets |  |  | 16.18 | % (c) |  |  | 18.57 | % (c)
Ratio of expense waiver/reimbursements to average net assets |  |  | (11.60 | %) (c) |  |  | (12.00 | %) (c)
Ratio of net expenses to average net assets |  |  | 4.58 | % (c) |  |  | 6.57 | % (c)
Ratio of net investment income to average net assets |  |  | 5.63 | % (c) |  |  | 0.79 | % (c)
PORTFOLIO TURNOVER RATE |  |  | 16.00 | % |  |  | 0.00 | %

(a) | Per share numbers have been calculated using the average shares method.

(b) | Based on net asset value per share. Distributions, if any, are assumed for purposes of this calculation to be reinvested at prices obtained under the Fund’s Dividend Reinvestment Plan. Total return is not annualized for periods less than one year.

(c) | Annualized (except organizational costs and non-recurring expenses).

See Notes to Financial Statements. |  |
Annual Report | March 31, 2026 |  | 13

Gladstone Alternative Income Fund |  | Financial Highlights - Class A
|  | March 31, 2026

|  | For the Year Ended March 31, 2026 |  |  | For the Period December 9, 2024 (Commencement of operations) to March 31, 2025 |
NET ASSET VALUE, BEGINNING OF PERIOD |  | $ | 10.00 |  |  | $ | 10.00 |
INCOME FROM INVESTMENT OPERATIONS |  |  |  |  |  |  |  |
Net investment income (a) |  |  | 0.62 |  |  |  | 0.05 |
Net realized and unrealized gain/(loss) on investments (a) |  |  | 0.05 |  |  |  | — |
Total income from investment operations (a) |  |  | 0.67 |  |  |  | 0.05 |
DISTRIBUTIONS |  |  |  |  |  |  |  |
From net investment income (a) |  |  | (0.69 | ) |  |  | (0.05 | )
Total distributions (a) |  |  | (0.69 | ) |  |  | (0.05 | )
NET ASSET VALUE, END OF PERIOD |  | $ | 9.98 |  |  | $ | 10.00 |
TOTAL RETURN (b) |  |  | 6.86 | % |  |  | 0.53 | %
RATIOS AND SUPPLEMENTAL DATA |  |  |  |  |  |  |  |
Net assets, end of period (000s) |  | $ | 613 |  |  | $ | 234 |
RATIOS TO AVERAGE NET ASSETS |  |  |  |  |  |  |  |
Ratio of gross expenses to average net assets |  |  | 15.82 | % (c) |  |  | 17.79 | % (c)
Ratio of expense waiver/reimbursements to average net assets |  |  | (11.54 | %) (c) |  |  | (11.95 | %) (c)
Ratio of net expenses to average net assets |  |  | 4.28 | % (c) |  |  | 5.84 | % (c)
Ratio of net investment income to average net assets |  |  | 6.23 | % (c) |  |  | 1.53 | % (c)
PORTFOLIO TURNOVER RATE |  |  | 16.00 | % |  |  | 0.00 | %

(a) | Per share numbers have been calculated using the average shares method.

(b) | Based on net asset value per share. Distributions, if any, are assumed for purposes of this calculation to be reinvested at prices obtained under the Fund’s Dividend Reinvestment Plan. Total return is not annualized for periods less than one year.

(c) | Annualized (except organizational costs and non-recurring expenses).

See Notes to Financial Statements. |  |
14 |  | www.gladstoneintervalfund.com

Gladstone Alternative Income Fund | Notes to Financial Statements

March 31, 2026
1. ORGANIZATION
Gladstone Alternative Income Fund (the “Fund”) is a
non-diversified,
closed-end
management investment company that is registered under the Investment Company Act of 1940, as amended (the “1940 Act”). In addition, for federal income tax purposes the Fund has elected to be treated, and intends to continue to maintain its qualification, as a regulated investment company (“RIC”) under Subchapter M of the Internal Revenue Code of 1986, as amended (the “Code”). The Fund is engaging in a continuous offering of its common shares of beneficial interests (“Shares”) and operates as an “interval fund” that makes quarterly offers to repurchase between 5% and 25% of its outstanding Shares at net asset value (“NAV”) per Share, reduced by any applicable repurchase fee. As of March 31, 2026, the Fund offered Class I Shares, Class A Shares, Class C Shares and Class U Shares and may offer additional classes of Shares in the future. Subject to applicable law and approval of the Fund’s Board of Trustees (the “Board” or “Board of Trustees,” and each of the trustees on the Board, a “Trustee”), for each quarterly repurchase offer, the Fund currently expects to offer to repurchase 5% of the outstanding Shares at NAV, which is the minimum amount permitted. The Fund determines the NAV of its Shares daily as of the close of regular trading (normally, 4:00 p.m., Eastern time) on each day that the New York Stock Exchange (“NYSE”) is open for business.
The Fund is externally managed by Gladstone Management Corporation (the “Adviser”), an entity that is registered with the U.S. Securities and Exchange Commission (the “SEC”) as an investment adviser under the Investment Advisers Act of 1940, as amended (the “Advisers Act”). Gladstone Administration, LLC (the “Administrator”) acts as the Fund’s administrator.
The Fund’s primary investment objective is to (i) achieve and grow current income by investing primarily in debt securities of established businesses or real estate holding intermediaries that the Fund believes will provide stable earnings and cash flow to pay expenses, make principal and interest payments on its outstanding indebtedness and make distributions to shareholders that grow over time; and (ii) provide the Fund’s shareholders with long-term capital appreciation in the value of its assets by investing in equity securities of established businesses, including in connection with the Fund’s debt investments, that the Fund believes can grow over time to permit the Fund to sell its equity investments for capital gains.
The Fund pursues its investment objective primarily by investing in directly originated loans to lower and middle market private businesses in the U.S., broadly syndicated loans and commercial real estate loans. The Fund also make equity investments, including in connection with its directly originated loans. The Fund seeks to avoid investing in high-risk,
pre-revenue,
early-stage enterprises. The Fund expects that most, if not all, of the debt securities it acquires will not be rated by a rating agency. Investors should assume that these loans would be rated below what is considered “investment grade” quality. Investments rated below investment grade are often referred to as high yield securities or junk bonds and may be considered higher risk as compared to investment grade debt instruments.
The Fund was organized as a statutory trust on May 29, 2024 under the laws of the State of Delaware. The Fund commenced operations on December 9, 2024 when it received investment proceeds for Shares beyond the initial seed capital and began actively seeking investment opportunities. The Fund is authorized to issue an unlimited number of Shares, which it offers through Gladstone Securities, LLC (the “Distributor”), an affiliated dealer manager, on a best-efforts basis. The initial NAV on December 9, 2024 was $10.00 for each Share class. The maximum sales load is 5.75% of the amount invested for Class A Shares. Class C Shares, Class I Shares and Class U Shares are not subject to an
up-front
sales charge. Class C Shares redeemed during the first 365 days after purchase may be subject to a contingent deferred sales charge equal to 1.00% of the invested amount. The minimum initial investment by a shareholder for Class I Shares, Class A Shares, Class C Shares and Class U

Annual Report | March 31, 2026 |  | 15

Gladstone Alternative Income Fund | Notes to Financial Statements

March 31, 2026
Shares are $250,000, $5,000, $5,000 and $5,000, respectively, per account. The Fund reserves the right to waive the minimum initial investment requirement for any investor. There is no minimum subsequent investment amount for any class of Shares.
2. SIGNIFICANT ACCOUNTING POLICIES
Basis of Presentation
The Fund prepares its financial statements and the accompanying notes in accordance with accounting principles generally accepted in the United States of America (“GAAP”) and they conform to SEC Regulation
S-X.
Management believes it has made all necessary adjustments so that its financial statements are presented fairly and that all such adjustments are of a normal recurring nature.
Consolidation
In accordance with Article 6 of SEC Regulation
S-X,
the Fund does not consolidate portfolio company investments. Under the investment company rules and regulations pursuant to the American Institute of Certified Public Accountants Audit and Accounting Guide for Investment Companies, codified in Financial Accounting Standards Board (“FASB”) Accounting Standards Codification (“ASC”) Topic 946 “Financial Services - Investment
Companies” (“ASC 946”), the Fund is precluded from consolidating any entity other than another investment company.
Use of Estimates
The preparation of financial statements in conformity with GAAP requires management to make estimates and assumptions related to the reported amounts of assets and liabilities and disclosure of contingent assets and liabilities at the date of the financial statements and the reported amounts of income and expenses during the period. Actual results could differ from those estimates.
Cash
Cash is carried at cost, which approximates fair value. The Fund places its cash with financial institutions, and, at times, cash deposit may exceed the Federal Deposit Insurance Corporation insured limit. The Fund seeks to mitigate this concentration of credit risk by depositing funds with major financial institutions.
Reverse Repurchase Agreements
Master Repurchase Agreements and Global Master Repurchase Agreements (individually and collectively “Master Repo Agreements”) govern repurchase, reverse repurchase, and certain sale-buyback transactions between the Fund and select counterparties. Master Repo Agreements maintain provisions for, among other things, initiation, income payments, events of default, and maintenance of collateral. The market value of transactions under the Master Repo Agreement, collateral pledged or received, and the net exposure by counterparty as of period end are disclosed in the Notes to Schedule of Investments. Under the Master Repo Agreement, the Fund enters into repurchase agreements under which the Fund transfers U.S. Treasury Bills to a counterparty in exchange for cash and simultaneously agrees to repurchase such securities at a fixed price on a specified future date. Repurchase agreements are accounted for as collateralized borrowings, as the Fund retains substantially all of the economic risks and rewards of ownership of the underlying securities. Accordingly, the U.S. Treasury Bills pledged as collateral remain recorded as investments, at fair value, and a corresponding liability is recorded as Payable for repurchase agreement, including accrued interest. Interest expense is recorded over the term of the agreement.

16 |  | www.gladstoneintervalfund.com

Gladstone Alternative Income Fund | Notes to Financial Statements

March 31, 2026
Organizational and Offering Costs
Organizational costs consist of costs incurred to establish the Fund and enable it legally to do business. Examples of these costs are fees paid to the Board of Trustees, legal fees, and audit fees relating to the seed audit. Organizational costs were charged to expenses as incurred. Offering costs incurred by the Fund were treated as deferred charges until operations commenced and thereafter were amortized over a
12-month
period using the straight-line method. Examples of these costs are registration fees, legal fees, and fees relating to the initial registration statement. All costs incurred by the Fund in connection with its organization and offering that are paid by the Adviser may be subject to reimbursement.
Federal Income Taxes
For federal income tax purposes, the Fund has elected to be treated, and intends to continue to maintain its qualification, as a RIC under Subchapter M of the Code.
As a RIC, the Fund generally will not be subject to corporate-level income taxes on any ordinary income or capital gains that the Fund distributes as dividends to its shareholders.
To qualify and maintain its qualification as a RIC, the Fund must, among other requirements, meet specified
source-of-income
and asset diversification requirements and distribute dividends to shareholders each taxable year of an amount generally at least equal to 90% of the Fund’s net ordinary taxable income and realized net short-term capital gains in excess of realized net long-term capital losses determined without regard to any deduction for dividends paid.
ASC 740, “Income Taxes” requires the evaluation of tax positions taken or expected to be taken in the course of preparing the tax returns to determine whether the tax positions are
“more-likely-than-not”
of being sustained by the applicable tax authorities. Tax positions not deemed to satisfy the
“more-likely-than-not”
threshold would be recorded as a tax benefit or expense in the current fiscal year. The Fund has evaluated the implications of ASC 740, for all open tax years and in all major tax jurisdictions, and determined that there is no material impact on the Fund’s accompanying Financial Statements.
If the Fund fails to distribute by the end of any calendar year an amount at least equal to the sum of (1) 98% of its ordinary income for the calendar year, (2) 98.2% of its capital gain net income (both long-term and short-term for the
one-year
period ending on October 31 of that calendar year and (3) any income realized, but not distributed, in the preceding year (to the extent that income tax was not imposed on such amounts) less certain over-distributions in prior years (together, the “Excise Tax Distribution Requirements”), the Fund will be liable for a 4% nondeductible excise tax on the
portion of the undistributed amounts of such income that are less than the amounts required to be distributed based on the Excise Tax Distribution Requirements. For this purpose, however, any ordinary income or capital gain net income retained by the Fund that is subject to corporate income tax for the tax year ending in that calendar year will be considered to have been distributed by year end (or earlier if estimated taxes are paid).
Distributions
Distributions to shareholders are recorded on the
ex-dividend
date. The Fund is required to distribute at least 90% of its investment company taxable income for each taxable year as a distribution to its shareholders to maintain its ability to be taxed as a RIC under Subchapter M of the Code. It is the Fund’s policy to generally pay out as a distribution up to 100% of those amounts. The amount to be paid is determined by the Board and is based upon management’s estimate of investment company taxable income, net long-term capital gains, as well as amounts

Annual Report | March 31, 2026 |  | 17

Gladstone Alternative Income Fund | Notes to Financial Statements

March 31, 2026
to be distributed in accordance with Section 855(a) of the Code. The Fund intends to continue to declare income dividends daily and distribute them to shareholders monthly. Based on that estimate, the Board declares such daily distributions on a monthly basis, and supplemental distributions, as applicable. At fiscal
year-end,
the Fund may elect to treat a portion of the first distributions paid after
year-end
as having been paid in the prior year in accordance with Section 855(a) of the Code. The Fund may retain some or all of its net long-term capital gains, if any, and designate them as deemed distributions, or distribute these capital gains to shareholders in cash. If the Fund elects to retain net long-term capital gains and deem them distributed, each U.S. common shareholder will be treated as if they received a distribution of their
pro-rata
share of the retained net long-term capital gain and the U.S. federal income tax paid. As a result, each common shareholder will (i) be required to report their
pro-rata
share of the retained gain on their tax return as long-term capital gain, (ii) receive a refundable tax credit for their
pro-rata
share of federal income tax paid by the Fund on the retained gain, and (iii) increase the tax basis of their shares of common shares by an amount equal to the deemed distribution less the tax credit.
Pursuant to the Fund’s dividend reinvestment plan, all shareholders will have all distributions, net of any applicable U.S. withholding tax, reinvested automatically in additional Shares of the same Class by SS&C GIDS, Inc., as agent for the shareholders, unless the shareholder elects to receive cash. Any distributions reinvested under the plan will be taxable to a common shareholder to the same extent, and with the same character, as if the common shareholder had received the distribution in cash. The common shareholder will have an adjusted basis in the additional common shares purchased through the plan equal to the dollar amount that would have been received if the U.S. shareholder had received the dividend or distribution in cash. The Fund issues Shares at NAV in connection with the obligations under the plan.
Segment Reporting
In November 2023, the FASB issued Accounting Standards Update
2023-07,
“Segment Reporting - Improvements to Reportable Segment Disclosures” (“ASU
2023-07”)
to improve reportable segments disclosure requirements. The ASU requires existing annual segment disclosures to also be disclosed on an interim basis and also requires additional disclosures around significant segment expenses and disclosures to identify the title and position of the chief operating decision maker (“CODM”). The standard is effective for fiscal years beginning after December 15, 2023, and interim periods thereafter. We adopted ASU
2023-07
as of September 30, 2025.
Our current business strategy includes one reporting segment which derives investment income from our portfolio companies. Our CODM is our president. The CODM assesses performance based on net investment income, net realized and unrealized gains (losses) and net increase (decrease) in net assets resulting from operations, which are reported on the Statement of Operations. The expense categories included on the Statement of Operations reflect our significant expense categories and are provided to the CODM on a regular basis.
The Fund uses the management approach to determine reportable operating segments. The management approach considers the internal organization and reporting used by the Fund’s chief operating decision maker (“CODM”) for making decisions, allocating resources, and assessing performance. The Fund’s CODM has been identified as the president of the Fund, who reviews results presented within the Fund’s financial statements when making decisions about allocating resources and assessing performance of the Fund. The CODM determined that the Fund has only one operating segment as defined by ASC Topic 280, “Segment Reporting”. This is supported by the single investment strategy of the Fund, against which the CODM assesses performance.

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Gladstone Alternative Income Fund | Notes to Financial Statements

March 31, 2026
Investment Valuation
Accounting Recognition
The Fund records its investments at fair value in accordance with the FASB ASC Topic 820, “Fair Value Measurement” (“ASC 820”) and the 1940 Act. Investment transactions are recorded on the trade date. Realized gains or losses are generally measured by the difference between the net proceeds from the repayment or sale and the cost basis of the investment, without regard to unrealized appreciation or depreciation previously recognized, and include investments charged off during the period, net of recoveries. Unrealized appreciation or depreciation primarily reflects the change in investment fair values, including the reversal of previously recorded unrealized appreciation or depreciation when gains or losses are realized.
Board Responsibility
The Board has approved investment valuation policies and procedures pursuant to Rule
2a-5
under the 1940 Act (the “Policy”) and designated the Adviser to serve as the Board’s valuation designee (“Valuation Designee”) under the 1940 Act.
In accordance with the 1940 Act, the Board has the ultimate responsibility for reviewing the good faith fair value determination of the Fund’s investments for which market quotations are not readily available based on the Policy and for overseeing the Valuation Designee. Such review and oversight includes receiving written fair value determinations and supporting materials provided by the Valuation Designee, in coordination with the Administrator and with the oversight by the Fund’s chief valuation officer (collectively, the “Valuation Team”). The Valuation Committee of the Board (comprised entirely of independent trustees) meets to review the valuation determinations and supporting materials, discusses the information provided by the Valuation Team, determines whether the Valuation Team has followed the Policy, and reviews other facts and circumstances, including current valuation risks, conflicts of interest, material valuation matters, appropriateness of valuation methodologies, back-testing results, price challenges/overrides, and ongoing monitoring and oversight of pricing services. After the Valuation Committee concludes its meeting, it and the chief valuation officer, representing the Valuation Designee, present the Valuation Committee’s findings on the Valuation Designee’s determinations to the entire Board so that the full Board may review the Valuation Designee’s determined fair values of such investments in accordance with the Policy.
There is no single standard for determining fair value (especially for privately-held businesses), as fair value depends upon the specific facts and circumstances of each individual investment. In determining the fair value of the Fund’s investments, the Valuation Team, led by the chief valuation officer, uses the Policy, and each quarter, at a minimum, the Valuation Committee and Board review the Policy to determine if changes thereto are advisable and whether the Valuation Team has applied the Policy consistently.
Use of Third Party Valuation Firms
The Valuation Team engages third-party valuation firms to provide independent assessments of fair value of certain of the Fund’s investments.
A third-party valuation firm generally provides estimates of fair value on the Fund’s debt investments. The Valuation Team generally assigns the third-party valuation firm’s estimates of fair value to debt investments where the Fund does not have the ability to effectuate a sale of the applicable portfolio company. The Valuation Team corroborates this third-party valuation firm’s estimates of fair value using one or more of the valuation techniques discussed below. The Valuation Team’s estimate of value on a specific debt investment may

Annual Report | March 31, 2026 |  | 19

Gladstone Alternative Income Fund | Notes to Financial Statements

March 31, 2026
significantly differ from the third-party valuation. When this occurs, the Valuation Committee and Board review whether the Valuation Team has followed the Policy and the Valuation Committee reviews whether the Valuation Designee’s determined fair value is reasonable in light of the Policy and other relevant facts and circumstances.
The Fund may engage other independent valuation firms to provide earnings multiple ranges, as well as other information, and evaluate such information for incorporation into the total enterprise value (“TEV”) of certain of the Fund’s investments. Generally, at least once per year, the Fund engages an independent valuation firm to value or review the valuation of each of the Fund’s significant equity investments, which includes providing the information noted above. The Valuation Team evaluates such information for incorporation into the Fund’s TEV, including review of all inputs provided by the independent valuation firm. The Valuation Team then presents a determination to the Valuation Committee as to the fair value. The Valuation Committee reviews the determined fair value and whether it is reasonable in light of the Policy and other relevant facts and circumstances.
Valuation Techniques
In accordance with ASC 820, the Valuation Team uses the following techniques when valuing the Fund’s investment portfolio:
Total
Enterprise
Value
— In determining the fair value using a TEV, the Valuation Team first calculates the TEV of the portfolio company by incorporating some or all of the following factors: the portfolio company’s ability to make payments and other specific portfolio company attributes; the earnings of the portfolio company (the trailing or projected twelve month revenue or EBITDA); EBITDA multiples obtained from the Fund’s indexing methodology whereby the original transaction EBITDA multiple at the time of closing is indexed to a general subset of comparable disclosed transactions and EBITDA multiples from recent sales to third parties of similar securities in similar industries; a comparison to publicly traded securities in similar industries; and other pertinent factors. The Valuation Team generally reviews industry statistics and may use outside experts when gathering this information. Once the TEV is determined for a portfolio company, the Valuation Team generally allocates the TEV to the portfolio company’s securities based on the facts and circumstances of the securities, which typically results in the allocation of fair value to securities based on the order of their relative priority in the capital structure. Generally, the Valuation Team uses TEV to value the Fund’s equity investments and, in the circumstances where the Fund has the ability to effectuate a sale of a portfolio company, the Fund’s debt investments. When there is equity value or sufficient TEV to cover the principal balance of debt securities, the fair value of senior secured debt generally equals or approximates cost.
TEV is primarily calculated using EBITDA and EBITDA multiples; however, TEV may also be calculated using revenue and revenue multiples or a discounted cash flow (“DCF”) analysis whereby future expected cash flows of the portfolio company are discounted to determine a net present value using estimated risk-adjusted discount rates, which incorporate adjustments for nonperformance and liquidity risks.
Yield Analysis
— The Valuation Team generally determines the fair value of debt investments for which the Fund does not have the ability to effectuate a sale of the applicable portfolio company using the yield analysis, which includes a DCF calculation and assumptions that the Valuation Team believes market participants would use, including, estimated remaining life, current market yield, current leverage, and interest rate spreads. This technique develops a modified discount rate that incorporates risk premiums including increased probability of default, increased loss upon default and increased liquidity risk. Generally, the Valuation Team uses the yield analysis to corroborate both estimates of value provided by the third party valuation firm and market quotes.

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Gladstone Alternative Income Fund | Notes to Financial Statements

March 31, 2026
Market Quotes
— For investments for which a limited market exists, the Fund generally bases fair value on readily available and reliable market quotations which are corroborated by the Valuation Team (generally by using the yield analysis described above). In addition, the Valuation Team assesses trading activity for similar investments and evaluates variances in quotations and other market insights to determine if any available quoted prices are reliable. Typically, the Valuation Team uses the lower indicative bid price (“IBP”) in the
bid-to-ask
price range obtained from the respective originating syndication agent’s trading desk on or near the valuation date. The Valuation Team may take further steps to consider additional information to validate that price in accordance with the Policy. For securities that are publicly traded, the Valuation Team generally bases fair value on the closing market price of the securities the Fund holds as of the reporting date. For restricted securities that are publicly traded, the Valuation Team generally bases fair value on the closing market price of the securities the Fund holds as of the reporting date less a discount for the restriction, which includes consideration of the nature and term to expiration of the restriction and the lack of marketability of the security.
For commercial real estate loans that trade/have a market, the valuation decision is based on the current market price. For commercial real estate loans that are not publicly traded, the Valuation Team will use another valuation technique that maximizes the use of relevant observable inputs and minimizes the use of unobservable inputs; fair value will be defined as the price that would be received to sell an asset or transfer a liability in an orderly transaction between market participants at a measurement date.
In addition to the valuation techniques listed above, the Valuation Team may also consider other factors when determining the fair value of the Fund’s investments, including: the nature and realizable value of the collateral, including external parties’ guaranties, any relevant offers or letters of intent to acquire the portfolio company, timing of expected loan repayments, and the markets in which the portfolio company operates.
Fair value measurements of the Fund’s investments may involve subjective judgments and estimates and due to the uncertainty inherent in valuing these securities, the determinations of fair value may fluctuate from period to period and may differ materially from the values that could be obtained if a ready market for these securities existed. The Fund’s NAV could be materially affected if the determinations regarding the fair value of investments are materially different from the values that are ultimately realized upon the Fund’s disposal of such securities. Additionally, changes in the market environment and other events that may occur over the life of the investment may cause the gains or losses ultimately realized on these investments to be different than the valuations currently assigned. Further, such investments are generally subject to legal and other restrictions on resale or otherwise are less liquid than publicly traded securities. If the Fund were required to liquidate a portfolio investment in a forced or liquidation sale, the Fund could realize significantly less than the value at which it is recorded.
Revenue and Expense Recognition
Daily Revenue and Expense Recognition
The Fund determines its NAV each business day based on daily accruals of revenue and expenses, utilizing forecasts of expected revenue and expense activity for the period.
Interest Income Recognition
Interest income, including the amortization of premiums, acquisition costs and amendment fees, the accretion of original issue discounts (“OID”), and
paid-in-kind
(“PIK”) interest, is recorded on the accrual basis to the extent that such amounts are expected to be collected. Generally, when a loan becomes 90 days or more past due or if the Fund’s qualitative assessment indicates that the debtor is unable to service its debt or other obligations, the

Annual Report | March 31, 2026 |  | 21

Gladstone Alternative Income Fund | Notes to Financial Statements

March 31, 2026
Fund will place the loan on
non-accrual
status and cease recognizing interest income on that loan for financial reporting purposes until the borrower has demonstrated the ability and intent to pay contractual amounts due. However, the Fund remains contractually entitled to this interest. Interest payments received on
non-accrual
loans may be recognized as income or applied to the cost basis depending upon management’s judgment. Generally,
non-accrual
loans are restored to accrual status when past due principal and interest are paid and, in management’s judgment, are likely to remain current, or due to a restructuring such that the interest income is deemed to be collectible.
The Fund expects to hold in the future some loans in the Fund’s portfolio that contain OID or PIK provisions. The Fund recognizes OID for loans originally issued at discounts and recognize the income over the life of the obligation based on an effective yield calculation. PIK interest, computed at the contractual rate specified in a loan agreement, is added to the principal balance of a loan and recorded as income over the life of the obligation. Thus, the actual collection of PIK income may be deferred until the time of debt principal repayment. To maintain the Fund’s ability to be taxed as a RIC, the Fund may need to pay out both OID and PIK
non-cash
income amounts in the form of distributions, even though the Fund has not yet collected the cash on either.
Success Fee Income Recognition
The Fund may enter into investments with success fees. The Fund records success fees as income when earned. Success fees are generally contractually due upon a change of control in a portfolio company, typically resulting from an exit or sale, and are
non-recurring.
Dividend Income Recognition
The Fund accrues dividend income on preferred and common equity securities to the extent that such amounts are expected to be collected and if the Fund has the option to collect such amounts in cash or other consideration. The money market fund generates revenue in the form of dividends, which are recorded on the
ex-dividend
date and included within “Dividend Income” in the Statement of Operations.
Related Party Fees
The Fund is party to an investment advisory agreement with the Adviser, dated November 6, 2024 (the “Advisory Agreement”), which is 100% indirectly owned by the chairman and chief executive officer. In accordance with the Advisory Agreement, the Fund pays the Adviser fees as compensation for its services, consisting of a base management fee and an incentive fee.
The Fund is also party to an administration agreement with the Administrator, dated November 6, 2024 (the “Administration Agreement”), which is indirectly owned by the chairman and chief executive officer, whereby the Fund pays separately for certain administrative services.
The Fund is party to a distribution agreement with the Distributor, dated November 6, 2024 (the “Distribution Agreement”), which is indirectly owned and controlled by the chairman and chief executive officer, whereby the Fund pays the Distributor to act as the distributor of Shares on a best-efforts basis.
In addition, under a distribution and servicing plan that the Fund has adopted, the Class C Shares and Class U Shares pay to the Distributor a distribution fee and the Class A Shares and Class C Shares pay the Distributor a servicing fee.

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Gladstone Alternative Income Fund | Notes to Financial Statements

March 31, 2026
Refer to Note 7 –
Management and Related Party Transactions
for additional information related to these related party fees and agreements.
Unfunded Commitments
The Fund may enter into unfunded loan commitments, which are contractual obligations for future funding, such as delayed draw term loans or revolving credit arrangements. Unfunded loan commitments represent a future obligation in full, even though a percentage of the notional loan amounts may not be utilized by the borrower. The Fund may receive a commitment fee based on the undrawn portion of the underlying line of credit portion of a floating rate loan. As of March 31, 2026, the Fund had unfunded commitments of $110,727. The Fund estimates the fair value of the combined unused line of credit commitments as of March 31, 2026 to be insignificant.
Recent Accounting Pronouncements
In December 2025, the FASB issued ASU
2025-11,
“Interim Reporting (Topic 270): Narrow-Scope Improvements”
(“ASU2025-11”),
which improves the navigability of required interim disclosures and clarifies when that guidance is applicable. Additionally, ASU
2025-11
provides additional guidance on what disclosures should be provided in interim reporting periods. ASU
2025-11
is effective for interim reporting periods within annual periods beginning after December 15, 2027. The Fund is currently assessing the impact of this guidance; however, the Fund does not expect a material impact on our financial statements.
In December 2025, the FASB issued ASU
2025-12,
“Codification Improvements” (“ASU
2025-12”),
which facilitates codification updates for a broad range of topics arising from technical corrections, unintended application of the codification, clarifications, and other minor improvements. ASU
2025-12
is effective for fiscal years beginning after December 15, 2026. The Fund is currently assessing the impact of this guidance; however, the Fund does not expect a material impact on our financial statements.
3. INVESTMENTS
Fair Value Measurements
In accordance with ASC 820, the fair value of the Fund’s investments are determined to be the price that would be received for an investment in a current sale, which assumes an orderly transaction between willing market participants on the measurement date. This fair value definition focuses on exit price in the principal, or most advantageous, market and prioritizes, within a measurement of fair value, the use of market-based inputs over entity-specific inputs. ASC 820 also establishes the following three-level hierarchy for fair value measurements based upon the transparency of inputs to the valuation of a financial instrument as of the measurement date.

| • |  | Level 1 – inputs to the valuation methodology are quoted prices (unadjusted) for identical financial instruments in active markets;

| • |  | Level 2 – inputs to the valuation methodology include quoted prices for similar financial instruments in active or inactive markets, and inputs that are observable for the financial instrument, either directly or indirectly, for substantially the full term of the financial instrument. Level 2 inputs are those in markets for which there are few transactions, the prices are not current, little public information exists, or instances where prices vary substantially over time or among brokered market makers; and

Annual Report | March 31, 2026 |  | 23

Gladstone Alternative Income Fund | Notes to Financial Statements

March 31, 2026

| • |  | Level 3 – inputs to the valuation methodology are unobservable and significant to the fair value measurement. Unobservable inputs are those inputs that reflect assumptions that market participants would use when pricing the financial instrument and can include the Valuation Team’s assumptions based upon the best available information.

When a determination is made to classify an investment within Level 3 of the valuation hierarchy, such determination is based upon the significance of the unobservable factors to the overall fair value measurement. However, Level 3 financial instruments typically include, in addition to the unobservable, or Level 3, inputs, observable inputs (or components that are actively quoted and can be validated to external sources). The level in the fair value hierarchy within which the fair value measurement falls is determined based on the lowest level input that is significant to the fair value measurement. As of March 31, 2026 and 2025, all of our investments were valued using Level 3 inputs within the ASC 820 fair value hierarchy, except for our investment in money market funds, which was valued using Level 1 inputs, and our investment in a United States Treasury Obligation, which was valued using Level 2 inputs.
The following is a summary of the inputs used to value the Fund’s investments as of March 31, 2026:

Investments in Securities at Value |  | Level 1 - Quoted Prices |  |  | Level 2 - Other Significant Observable Inputs |  |  | Level 3 - Significant Unobservable Inputs |  |  | Total |
Debt Investments |  | $ | — |  |  | $ | — |  |  | $ | 14,369,497 |  |  | $ | 14,369,497 |
Equity Investments |  |  | — |  |  |  | — |  |  |  | 2,608,366 |  |  |  | 2,608,366 |
Short Term Investments |  |  | 3,697,074 |  |  |  | 8,999,099 |  |  |  | — |  |  |  | 12,696,173 |
Total |  | $ | 3,697,074 |  |  | $ | 8,999,099 |  |  | $ | 16,977,863 |  |  | $ | 29,674,036 |
Other Financial Instruments |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |
Liabilities: |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |
Reverse Repurchase Agreements |  | $ | — |  |  | $ | (8,100,000 | ) |  | $ | — |  |  | $ | (8,100,000 | )
Total |  | $ | — |  |  | $ | (8,100,000 | ) |  | $ | — |  |  | $ | (8,100,000 | )

The following is a reconciliation of assets in which Level 3 inputs were used in determining value:

Asset Type |  | Balance as of March 31, 2025 |  |  | Change in Unrealized Appreciation/ Depreciation |  |  | Purchases |  |  | Sales Proceeds |  |  | Balance as of March 31, 2026 |  |  | Net change in unrealized appreciation/ (depreciation) included in the Statements of Operations attributable to Level 3 investments held at March 31, 2026 |
Debt Investments |  | $ | 11,460,120 |  |  | $ | 7,507 |  |  | $ | 5,353,841 |  |  | $ | (2,451,971 | ) |  | $ | 14,369,497 |  |  | $ | 7,507 |
Equity Investments |  |  | 1,778,597 |  |  |  | 49,817 |  |  |  | 867,150 |  |  |  | (87,198 | ) |  |  | 2,608,366 |  |  |  | 49,817 |
|  | $ | 13,238,717 |  |  | $ | 57,324 |  |  | $ | 6,220,991 |  |  | $ | (2,539,169 | ) |  | $ | 16,977,863 |  |  | $ | 57,324 |

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Gladstone Alternative Income Fund | Notes to Financial Statements

March 31, 2026
In accordance with ASC 820, the following table provides quantitative information about the Fund’s Level 3 fair value measurements of its investments as of March 31, 2026. The table below is not intended to be
all-inclusive,
but rather provides information on the significant Level 3 inputs as they relate to fair value measurements. The weighted average calculations in the table below are based on the principal balances for all debt related calculations and on the cost basis for all equity related calculations for the particular input.

|  | Quantitative Information about Level 3 Fair Value Measurements
|  | Fair Value as of |  |  | Valuation Technique / |  | Unobservable Input |  | Range / Weighted- Average as of
|  | March 31, 2026 |  |  | March 31, 2026
Secured first lien debt |  | $ | 10,697,627 |  |  | Yield Analysis |  | Discount Rate |  | 10.7% -13.1% / 12.2%
Secured second lien debt |  |  | 3,671,870 |  |  | Yield Analysis |  | Discount Rate |  | 11.2% - 12.3% / 11.5%
|  |  |  |  |  |  |  | EBITDA |  |
Preferred and common equity / equivalents |  |  | 2,608,366 |  |  | TEV |  | multiple |  | 4.3x - 7.0x / 5.8x
|  |  |  |  |  | TEV |  | EBITDA |  | $7,159 - $37,431/ $13,179
Total Level 3 Investments, at Fair Value |  | $ | 16,977,863 |  |  |  |  |  |  |

Fair value measurements can be sensitive to changes in one or more of the valuation inputs. Changes in discount rates, EBITDA, or EBITDA multiples (or revenue or revenue multiples), each in isolation, may change the fair value of certain of our investments. Generally, an increase/(decrease) in market yields or discount rates or a (decrease)/increase in EBITDA or EBITDA multiples (or revenue or revenue multiples) may result in a (decrease)/ increase in the fair value of certain of our investments.
4. TAX BASIS INFORMATION
As of March 31, 2026, the components for distributable earnings on a tax basis were as follows:

|  | Undistributed Ordinary Income |  |  | Accumulated Capital Gains/ (Losses) |  |  | Unrealized Appreciation/ (Depreciation) |  |  | Other Cumulative Effect of Timing Differences |  |  | Total |
Gladstone Alternative Income Fund |  | $ | 9,218 |  |  | $ | — |  |  | $ | 64,233 |  |  | $ | (55,473 | ) |  | $ | 17,978 |

The tax character of distributions paid by the Fund for the fiscal year ended March 31, 2026 were as follows:

|  | Ordinary Income |  |  | Return of Capital |
Gladstone Alternative Income Fund |  | $ | 1,416,055 |  |  | $ | — |

The tax character of distributions paid by the Fund for the fiscal year ended March 31, 2025 were as follows:

|  | Ordinary Income |  |  | Return of Capital |
Gladstone Alternative Income Fund |  | $ | 106,633 |  |  | $ | — |

Annual Report | March 31, 2026 |  | 25

Gladstone Alternative Income Fund | Notes to Financial Statements

March 31, 2026
As of March 31, 2026, the aggregate cost of investments, gross unrealized appreciation/(depreciation) and net unrealized depreciation for Federal income tax purposes were as follows:

|  | Cost of Investments for Federal Income Tax Purposes |  |  | Gross Appreciation (Excess of Value Over Tax Cost) |  |  | Gross Depreciation (Excess of Tax Cost over Value) |  |  | Net Appreciation/ (Depreciation) of Foreign Currency |  |  | Net Unrealized Appreciation/ (Depreciation) |
Gladstone Alternative Income Fund |  | $ | 29,609,803 |  |  | $ | 889,903 |  |  | $ | (825,670 | ) |  | $ | — |  |  | $ | 64,233 |

In December 2023, the FASB issued ASU
2023-09,
“Income Taxes (Topic 740): Improvements to Income Tax Disclosures” which was issued to enhance the transparency and decision usefulness of income tax disclosures. The new guidance is effective for annual periods beginning after December 15, 2024. We have adopted ASU
2023-09
effective as of April 1, 2025 and concluded the application of this guidance did not have a material effect on our financial statements.
5. SECURITIES TRANSACTIONS
Purchases and sales of securities, excluding short-term securities, during the year ended March 31, 2026 were as follows:

Fund |  | Purchases of Securities |  |  | Proceeds From Sales of Securities |
Gladstone Alternative Income Fund |  | $ | 6,220,991 |  |  | $ | 2,451,971 |

6. REPURCHASE OFFERS
The Fund is a
closed-end
“interval” fund. The Fund has adopted, pursuant to Rule
23c-3
under the 1940 Act, a fundamental policy, which cannot be changed without the approval of the holders of a majority of the Fund’s outstanding common shares of beneficial interest, requiring the Fund to offer to repurchase at least 5% and up to 25% of the Fund’s outstanding shares at NAV on a regular schedule.
The repurchase price will be the Fund’s NAV per Share as determined at the close of business on a date that will generally be the same date as the repurchase offer ends, but that may be up to fourteen (14) calendar days following such date, or on the next business day if the fourteenth day is not a business day.
Although the Fund’s policy permits repurchases of between 5% and 25% of the Fund’s outstanding Shares, for each quarterly repurchase offer, the Fund currently expects to offer to repurchase 5% of the Fund’s outstanding common shares at NAV, subject to Board approval. Repurchase offers in excess of 5% are made solely at the discretion of the Board and investors should not rely on any expectation of repurchase offers in excess of 5%. Even though the Fund makes quarterly repurchase offers, investors should consider the Shares illiquid.
During the year ended March 31, 2026, the Fund completed four repurchase offers. In these offers, the Fund offered to repurchase up to 5% of the number of its outstanding shares as of the Repurchase Pricing Dates. The result of the repurchase offer is as follows:

Commencement Date of Offer |  | May 1, 2025 |  | August 1, 2025 |  | October 31, 2025 |  | January 30, 2026
Repurchase Request Deadline |  | June 2, 2025 |  | September 2, 2025 |  | December 1, 2025 |  | February 27, 2026
Repurchase Pricing Date |  | June 2, 2025 |  | September 2, 2025 |  | December 1, 2025 |  | February 27, 2026
Amount Repurchased |  | $— |  | $— |  | $— |  | $—
Shares Repurchased |  | $— |  | $— |  | $— |  | $—

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Gladstone Alternative Income Fund | Notes to Financial Statements

March 31, 2026
7. MANAGEMENT AND RELATED PARTY TRANSACTIONS
Investment Advisory Agreement
Pursuant to the Advisory Agreement by and between the Fund and the Adviser, the Adviser is entitled to receive a fee consisting of two components — a base management fee and an incentive fee.
The base management fee is payable monthly in arrears and is calculated at an annual rate of 1.25% accrued daily based upon the Fund’s average daily net assets during such period. “Net assets” means the total value of all of the Fund’s assets, less an amount equal to all of the Fund’s accrued debts, liabilities and obligations and before taking into account any management or incentive fees payable or contractually due but not payable during the period. These calculations will be appropriately prorated for any period of less than a month. For the year ended March 31, 2026, the Adviser earned base management fees of $259,508, of which $117,169 was permanently offset by credits without recourse by the Adviser.
The incentive fee is calculated and payable quarterly in arrears in an amount equal to 15% of the Fund’s
“pre-incentive
fee net investment income” for the immediately preceding calendar quarter. The Fund’s
pre-incentive
fee net investment income, expressed as a rate of return on the Fund’s net assets as of the end of the immediately preceding quarter, is compared to a hurdle rate of 1.75% per quarter (7.00% annualized), subject to a
“catch-up”
feature. For these purposes,
“pre-incentive
fee net investment income” means interest income, dividend income and any other income (including any other fees, such as commitment, origination, structuring, diligence, and consulting fees that the Fund receives from portfolio companies) accrued by the Fund during the calendar quarter, minus the Fund’s operating expenses for the quarter (including the base management fee (less any rebate of other fees received by the Adviser), expenses payable under the Administration Agreement and any interest expenses and dividends paid on any issued and outstanding preferred shares, but excluding the incentive fee and any distributions and/ or shareholder servicing fees).
Pre-incentive
fee net investment income includes, in the case of investments with a deferred interest feature (such as original issue discount, debt instruments with
payment-in-kind
interest and
zero-coupon
securities), accrued income that the Fund has not yet received in cash.
Pre-incentive
fee net investment income does not include any realized capital gains, realized capital losses or unrealized capital appreciation or depreciation. For the year ended March 31, 2026, the Adviser did not earn any incentive fees.
Expense Support and Conditional Reimbursement Agreement
The Adviser and the Fund entered into the Expense Support and Conditional Reimbursement Agreement (dated October 8, 2024) (the “Expense Support Agreement”) under which the Adviser agreed contractually for a
one-year
period to reimburse the Fund’s initial organizational and offering costs, as well as the operating expenses of each Class of Shares commencing with the first quarter following the date of the Fund’s prospectus, to the extent that aggregate distributions made to its shareholders of a Class during the applicable quarter exceed Available Operating Funds (as defined below). On October 30, 2025, the Adviser and the Fund extended the Expense Support Agreement for an additional
one-year
term. The Expense Support and Conditional Reimbursement Agreement will remain in effect until the end of such term unless and until the Fund’s Board approves its modification or termination. On April 28, 2026, the Adviser and the Fund renewed the Expense Support Agreement. See Note 10 for further details on a subsequent extension of the Expense Support Agreement. Additionally, during the term of the Expense Support Agreement, the Adviser may reimburse the Fund’s or any Class’s operating expenses to the extent that it otherwise deems appropriate to ensure that the Fund or such Class bear an appropriate level of expenses (each such payment, an “Expense Payment”). “Available Operating Funds” means the sum of (i) the Fund’s net investment company taxable income (including net
short-term
capital gains reduced by net long-term capital losses); (ii) the Fund’s net capital gains (including the excess of net long-term capital gains over net short-term capital losses); and (iii) dividends and other

Annual Report | March 31, 2026 |  | 27

Gladstone Alternative Income Fund | Notes to Financial Statements

March 31, 2026
distributions paid to or otherwise earned by us on account of investments in portfolio companies (to the extent such amounts listed in clause (iii) are not included under clauses (i) and (ii) above).
In consideration of the Adviser’s agreement to reimburse the Fund’s operating expenses, the Fund has agreed to repay the Adviser in the amount of any of its expenses reimbursed, subject to the limitation that a reimbursement (an “Adviser Reimbursement”) will be made only if and to the extent that (i) it is payable not more than three years from the date on which the applicable Expense Payment was made by the Adviser; (ii) the Adviser Reimbursement does not cause Other Fund Operating Expenses (as defined below) attributable to Shares of such Class (on an annualized basis and net of any reimbursements received by the Fund with respect to such Class during such fiscal year) during the applicable quarter to exceed the percentage of average net assets attributable to Shares of such Class represented by Other Fund Operating Expenses (on an annualized basis) during the fiscal quarter in which the applicable Expense Payment from the Adviser was made; (iii) the Adviser Reimbursement does not cause the Fund to breach any other expense cap in place at the time of such Adviser Reimbursement; and (iv) the distributions per share declared by the Fund for the applicable Class at the time of the applicable Expense Payment are less than the effective rate of distributions per share for such Class of Shares at the time the Adviser Reimbursement would be paid.
Other Fund Operating Expenses is defined as the Fund’s total Operating Expenses (as defined below), excluding the management and incentive fees payable to the Adviser, any offering expenses, financing fees and costs, interest expense, distribution fees, shareholder servicing fees and extraordinary expenses. “Operating Expenses” means all operating costs and expenses the Fund incurs, as determined in accordance with generally accepted accounting principles for investment companies. Additionally, the Adviser paid expenses on behalf of the Fund, which are reimbursed by the Fund to the Adviser in the following quarter. At March 31, 2026, outstanding reimbursable expenses totaled $30,549.
For the year ended March 31, 2026, the Adviser waived fees and reimbursed expenses totaling $2,304,650.
As of March 31, 2026, the balance of recoupable expenses was as follows:

|  | As of March 31, 2026 |
Expiration Period |  |  |
Less than 1 year |  | $ | — |
1-2 years |  |  | 1,096,997 |
2-3 years |  |  | 2,304,650 |
Total |  | $ | 3,401,647 |

Administrative and Other Service Providers
The Administrator provides certain administrative services to the Fund pursuant to the Administration Agreement, by and between the Fund and the Administrator. The Fund reimburses the Administrator pursuant to the Administration Agreement for its allocable portion of the Administrator’s expenses incurred while performing services to the Fund, which are primarily rent and salaries and benefits expenses of the Administrator’s employees, including its chief financial officer and treasurer, chief valuation officer, chief compliance officer, chief administrative officer and
co-general
counsels and
co-secretaries,
and their respective staffs. One of the Fund’s executive officers, David Gladstone (its chairman and chief executive officer) serves as a member of the board of managers and executive officer of the Administrator, which is 100% indirectly owned and controlled by Mr. Gladstone. Michael LiCalsi, the Fund’s chief administrative officer,
co-general
counsel and
co-secretary,
also serves in the same roles for the Administrator (in addition to serving as president of the Administrator). Mr. Hellmold, the Fund’s
co-general
counsel and
co-secretary,
also serves in the same roles for the Administrator.

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Gladstone Alternative Income Fund | Notes to Financial Statements

March 31, 2026
The Fund’s allocable portion of the Administrator’s expenses is generally derived by multiplying the Administrator’s total expenses by the approximate percentage of time during the applicable quarter the Administrator’s employees performed services for the Fund in relation to their time spent performing services for all companies serviced by the Administrator. The Administration Agreement has an initial
two-year
term which expires November 23, 2026 and is subject to annual renewal by the board of trustees, including a majority of the trustees who are not parties to the Administration Agreement or interested persons of either party.
Administration fees for the year ended March 31, 2026 were $848,924.
ALPS Fund Services, Inc. (“ALPS”) and certain of its affiliates provide
sub-administrative,
fund accounting and other services to the Fund for a monthly administration fee based on the greater of an annual minimum fee or an asset-based fee, which scales downward based upon average daily net assets.
UMB Bank, n.a. (“Custodian”), serves as the Fund’s custodian.
The Distributor acts as an agent for the Fund and the distributor of its Shares. Under the distribution agreement between the Fund and the Distributor, the Distributor may receive up to 5.75% of the investment amount of the Fund’s Class A Shares as an upfront sales charge. No upfront sales charge will be payable on the other Classes of the Fund’s Shares under the Distribution Agreement.
In addition, under a distribution and servicing plan that the Fund has adopted, the Class C Shares and Class U Shares pay to the Distributor a distribution fee that will accrue at an annual rate equal to 0.75% of the average daily net assets attributable to Class C Shares and Class U Shares, respectively, and is payable on a quarterly basis. Under the plan, the Class A Shares and Class C Shares pay to the Distributor a servicing fee that will accrue at an annual rate equal to 0.25% of the average daily net assets attributable to the Class A Shares and Class C Shares, respectively.
SS&C GIDS, Inc. serves as the Transfer Agent to the Fund and is responsible for maintaining all shareholder records of the Fund.
8. BORROWINGS AND OTHER FINANCING TRANSACTIONS
Borrowings
On October 2, 2025, the Fund entered into a Senior Secured Credit Agreement (the “Credit Facility”) with PNC Bank, National Association, establishing a line of credit that allows the Fund to borrow up to $5.0 million with an “accordion” feature that permits the Fund to increase the size of the facility to $50.0 million. The Credit Facility will mature on October 2, 2028. The Credit Facility bears an interest rate equal to Daily Simple SOFR plus a 3.0% margin, and has an unused commitment fee of 0.75% to 0.50%, depending on the amount of unused commitment.
The Credit Facility contains covenants that require the Fund to maintain its status as a separate legal entity, prohibit certain significant corporate transactions (such as mergers, consolidations, liquidations or dissolutions), and restrict certain modifications to its investment and valuation policies without the lender’s consent. Our Credit Facility also generally limits further indebtedness, liens against our assets, fundamental changes in and dispositions of assets, and limits distributions to our shareholders on a fiscal year basis to the sum of our net investment income, net capital gains and amounts elected to have been paid during the prior year in accordance with Section 852 of the Code. The Fund is also subject to certain limitations on the type of loan investments it

Annual Report | March 31, 2026 |  | 29

Gladstone Alternative Income Fund | Notes to Financial Statements

March 31, 2026
can apply as collateral towards the borrowing base to receive additional borrowing availability under our Credit Facility, including restrictions on borrower concentration,
non-U.S.
or foreign currency concentration, sector concentration, loan type and loan performance. Our Credit Facility further requires the Fund to comply with other financial and operational covenants, which obligate the Fund to, among other things, maintain a minimum
asset-to-senior
debt coverage ratio of 3.00 to 1.00.
Additionally, the Fund is required to maintain the status as an investment company under the 1940 Act and as a RIC under Subchapter M of the Internal Revenue Code of 1986, as amended.
As of March 31, 2026, the Fund had no borrowings outstanding under the Credit Facility and was in compliance with all of our Credit Facility covenants. Interest expense is recorded over the term of the agreement. The Fund incurred $15,000 of fees related to the Credit Facility which is included in interest expense on the Statement of Operations.
Other Financing Transactions
On March 27, 2026, the Fund entered into a reverse repurchase agreement transaction with the Custodian to borrow $8,100,000 in connection with the purchase of $9,000,000 of U.S. Treasury bills. The interest rate on the borrowings was 4.4% and the scheduled maturity was April 2, 2026. See Note 10 for further information. The fair value of the Reverse Repurchase Agreements as of March 31, 2026 was $8,101,980. The Fund considers the Reverse Repurchase Agreements to be Level 2 within the ASC 820 fair value hierarchy.
Under the Master Repo Agreement, the Fund enters into repurchase agreement transactions under which the Fund transfers U.S. Treasury Bills to a counterparty in exchange for cash and simultaneously agrees to repurchase such securities at a fixed price on a specified future date. Repurchase agreements are accounted for as collateralized borrowings, as the Fund retains substantially all of the economic risks and rewards of ownership of the underlying securities. Accordingly, the U.S. Treasury Bills pledged as collateral remain recorded as investments, at fair value, and a corresponding liability is recorded as Payable for repurchase agreement, including accrued interest. Interest expense is recorded over the term of the agreement. The Fund incurred $15,812 of interest expense related to reverse repurchase transactions which is included in interest expense on the Statement of Operations.

Weighted average interest rate at end of the period |  |  | 4.77 | %
Maximum aggregate balance outstanding at any time during the period |  | $ | 9,000,000 |
Average balance outstanding during the period |  | $ | 298,356 |
Average interest rate during the period |  |  | 4.76 | %

At March 31, 2026, interest rate on reverse repurchase agreement was 4.40% and the maturity was April 2, 2026. The interest rate on collateral was 3.46% and the maturity date was April 2, 2026. The outstanding agreements to repurchase as of
period-end
may be called by the counterparty before their maturity dates.
At March 31, 2026, investment securities with fair values amounting to $8,548,269 are pledged as collateral for reverse repurchase agreements. The counterparties have the right to sell or repledge the assets during the term of the reverse repurchase agreement with the Fund. Interest payable on reverse repurchase agreements amounted to $1,980 at March 31, 2026.

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Gladstone Alternative Income Fund | Notes to Financial Statements

March 31, 2026
9. INDEMNIFICATIONS
The Fund indemnifies its officers and trustees, as well as the Adviser, Administrator and Distributor for certain liabilities that may arise from the performance of their duties to the Fund. Additionally, in the normal course of business, the Fund enters into contracts that contain a variety of representations and warranties and which provide general indemnities. The Fund’s maximum exposure under these arrangements is unknown, as this would involve future claims that may be made against the Fund that have not yet occurred. However, the Fund expects the risk of loss due to these warranties and indemnities to be remote.
10. SUBSEQUENT EVENTS
Subsequent events after the date of the Statement of Assets and Liabilities have been evaluated through the date the financial statements were issued. Based on the evaluation, no adjustments were required to the financial statements as of March 31, 2026.
On April 2, 2026, the Fund’s $9,000,000 investment in the United States Treasury Bill matured. The proceeds were used on that same date to settle the payable for the reverse repurchase agreement.
On April 28, 2026, the Adviser and the Fund renewed the Expense Support Agreement discussed in Note 7 for an additional
one-year
term, expiring November 6, 2027.

Annual Report | March 31, 2026 |  | 31

Gladstone Alternative Income Fund | Report of Independent Registered Public Accounting Firm

To the Board of Trustees and Shareholders of Gladstone Alternative Income Fund
Opinion on the Financial Statements
We have audited the accompanying statement of assets and liabilities, including the schedule of investments, of Gladstone Alternative Income Fund (the “Fund”) as of March 31, 2026 and the related statements of operations and cash flows, for the year ended March 31, 2026 and the statement of changes in net assets and the financial highlights for the year ended March 31, 2026 and for the period December 9, 2024 (commencement of operations) to March 31, 2025, including the related notes (collectively referred to as the “financial statements”).
In our opinion, the financial statements present fairly, in all material respects, the financial position of the Fund as of March 31, 2026, the results of its operations and its cash flows for the year ended March 31, 2026, and the changes in its net assets and the financial highlights for the year ended March 31, 2026 and for the period December 9, 2024 (commencement of operations) to March 31, 2025 in conformity with accounting principles generally accepted in the United States of America.
Basis for Opinion
These financial statements are the responsibility of the Fund’s management. Our responsibility is to express an opinion on the Fund’s financial statements based on our audits. We are a public accounting firm registered with the Public Company Accounting Oversight Board (United States) (PCAOB) and are required to be independent with respect to the Fund in accordance with the U.S. federal securities laws and the applicable rules and regulations of the Securities and Exchange Commission and the PCAOB.
We conducted our audits of these financial statements in accordance with the standards of the PCAOB. Those standards require that we plan and perform the audit to obtain reasonable assurance about whether the financial statements are free of material misstatement, whether due to error or fraud.
Our audits included performing procedures to assess the risks of material misstatement of the financial statements, whether due to error or fraud, and performing procedures that respond to those risks. Such procedures included examining, on a test basis, evidence regarding the amounts and disclosures in the financial statements. Our audits also included evaluating the accounting principles used and significant estimates made by management, as well as evaluating the overall presentation of the financial statements. Our procedures included confirmation of securities owned as of March 31, 2026 by correspondence with the custodian, agent banks, and portfolio company investees. We believe that our audits provide a reasonable basis for our opinion.
/s/ PricewaterhouseCoopers LLP
Washington, District of Columbia
May 27, 2026
We have served as the auditor of the Fund since 2024.

32 |  | www.gladstoneintervalfund.com

Gladstone Alternative Income Fund |  | Additional Information (Unaudited)
|  | March 31, 2026

PROXY VOTING POLICIES AND VOTING RECORD
A description of the policies and procedures that the Fund uses to determine how to vote proxies relating to portfolio securities is available without charge, upon request, (i) by calling the Fund (toll-free) at (877)
774-7724,
or (ii) on the website of the SEC at http://www.sec.gov.
Information regarding how the Fund voted proxies relating to portfolio securities during the most recent
12-month
period ended June 30 is available (i) without charge, upon request, by calling the Fund (toll-free) at (877)
774-7724,
or (ii) on the SEC’s website at http://www.sec.gov.
PORTFOLIO HOLDINGS
The Fund’s portfolio holdings are made available semi-annually in shareholder reports within 60 days after the close of the period for which the report is being made, as required by federal securities laws. The Fund files its complete schedule of portfolio holdings with the SEC for the first and third fiscal quarters of each fiscal year as an exhibit to its reports on Form
N-PORT.
The Fund’s Form
N-PORT
reports are available on the SEC’s website at www.sec.gov. The Fund’s portfolio holdings are also available upon request, without charge, by calling (toll-free) (877)
774-7724,
or by writing to the Fund at c/o SS&C GIDS, Inc at P.O. Box 219597, Kansas City, MO 64121-9597.
TRUSTEE COMPENSATION
The following table sets forth certain information with respect to the compensation of each Trustee paid for the fiscal year ended March 31, 2026:

|  | Aggregate Regular Compensation From the Fund |  |  | Aggregate Special Compensation From the Fund |  |  | Total Compensation From the Fund |
David Gladstone* |  | $ | — |  |  | $ | — |  |  | $ | — |
Paula Novara* |  |  | — |  |  |  | — |  |  |  | — |
Michela A. English |  |  | 10,750 |  |  |  | — |  |  |  | 10,750 |
Katharine C. Gorka |  |  | 11,000 |  |  |  | — |  |  |  | 11,000 |
John H. Outland |  |  | 14,500 |  |  |  | — |  |  |  | 14,500 |
Anthony W. Parker |  |  | 13,625 |  |  |  | — |  |  |  | 13,625 |
Walter H. Wilkinson, Jr. |  |  | 12,500 |  |  |  | — |  |  |  | 12,500 |
Total |  | $ | 62,375 |  |  | $ | — |  |  | $ | 62,375 |

* | Mr. Gladstone and Ms. Novara are interested persons of the Fund, within the meaning of Section 2(a)(19) of the 1940 Act, due to their positions as officers of the Fund and of the Adviser and their employment by the Adviser.

Officers who are employed by the Adviser receive no compensation or expense reimbursement from the Fund.

Annual Report | March 31, 2026 |  | 33

Gladstone Alternative Income Fund | Trustees & Officers (Unaudited)

March 31, 2026
The following tables present certain information regarding the Board of Trustees and our officers. The address of each Trustee and officer as it relates to our business is 1521 Westbranch Drive, Suite 100, McLean, Virginia 22102. Each Trustee and officer serves for an indefinite term (i.e., until his or her death, resignation, retirement, or removal).

Name (Age) |  | Position Held (Length of Time Served) |  | Principal Occupation (s) During Past 5 Years |  | Number of Portfolios in Fund Complex (1) Overseen by Trustee |  | Other Directorships Held During Past 5 Years
INTERESTED TRUSTEES (2)
David Gladstone (83) |  | Trustee, Chairman, Chief Executive Officer (since inception) |  | Founder, Chief Executive Officer of Gladstone Investment Corporation (“Gladstone Investment”) since its inception in 2005 until March 2026, of Gladstone Capital Corporation (“Gladstone Capital”) since its inception in 2001 until March 2026, of Gladstone Commercial Corporation (“Gladstone Commercial”) since its inception in 2003 until March 2026, and of Gladstone Land Corporation (“Gladstone Land”) since its inception in 1997 until March 2026. Chairman of the Board of Gladstone Investment since its inception in 2005, of Gladstone Capital since its inception in 2001, of Gladstone Commercial since its inception in 2003, of Gladstone Land since its inception in 1997. Founder, Chief Executive Officer and Chairman of the Board |  | 3 |  | Gladstone Capital; Gladstone Investment; Gladstone Commercial; Gladstone Land; Gladstone Acquisition

34 |  | www.gladstoneintervalfund.com

Gladstone Alternative Income Fund | Trustees & Officers (Unaudited)

March 31, 2026

Name (Age) |  | Position Held (Length of Time Served) |  | Principal Occupation (s) During Past 5 Years |  | Number of Portfolios in Fund Complex (1) Overseen by Trustee |  | Other Directorships Held During Past 5 Years
INTERESTED TRUSTEES (2)
|  |  |  | of the registrant since 2024. Founder, Chief Executive Officer and Chairman of the Board of our Adviser. Since 2010, Mr. Gladstone also serves on the board of managers of Gladstone Securities, LLC (the “Distributor”). Chief Executive Officer, President, Chief Investment Officer. Chief Executive Officer, President, Chief Investment Officer and Director of Gladstone Acquisition Corporation from January 2021 until October 2022. |  |  |  |
Paula Novara (57) |  | Trustee; Head of Resource Management (since 2024) |  | Head of Human Resources, Facilities & Office Management and IT at Gladstone Capital, Gladstone Investment, Gladstone Commercial, Gladstone Land and the Fund since 2001, 2005, 2003, 1997, and 2024, respectively. |  | 3 |  | Gladstone Capital; Gladstone Investment; Gladstone Commercial; Gladstone Land

Annual Report | March 31, 2026 |  | 35

Gladstone Alternative Income Fund | Trustees & Officers (Unaudited)

March 31, 2026

INDEPENDENT TRUSTEES
Name (Age) |  | Position Held (Length of Time Served) |  | Principal Occupation (s) During Past 5 Years |  | Number of Portfolios in Fund Complex (1) Overseen by Trustee |  | Other Directorships Held During Past 5 Years
Michela A. English (76) |  | Trustee (since 2024) |  | Private Investor and Director who has served on multiple corporate and non-profit boards. |  | 3 |  | Gladstone Capital; Gladstone Investment; Gladstone Commercial; Gladstone Land; Gladstone Acquisition
Katharine C. Gorka (65) |  | Trustee (since 2024) |  | President of Threat Knowledge Group, which provides training and expertise on threats to U.S. national security, since 2010, President of Revere Pay, Inc., and the chair of the Fairfax County Republican Party since 2024; senior policy advisor in the Office of Policy at the Department of Homeland Security from 2017 to 2020; press secretary for U.S. Customs and Border Protection in 2020; and Director for Civil Society at The Heritage Foundation from 2020 to 2022. |  | 3 |  | Gladstone Capital; Gladstone Investment; Gladstone Commercial; Gladstone Land
John H. Outland (80) |  | Trustee (since 2024) |  | Private investor since June 2006. |  | 3 |  | Gladstone Capital; Gladstone Investment; Gladstone Commercial; Gladstone Land; Gladstone Acquisition
Anthony W. Parker (80) |  | Trustee (since 2024) |  | Founder and former chairman of the Board of Parker Tide Corp., a government contracting company. Former Treasurer of the Republican |  | 3 |  | Gladstone Capital; Gladstone Investment; Gladstone Commercial; Gladstone Land; Gladstone Acquisition

36 |  | www.gladstoneintervalfund.com

Gladstone Alternative Income Fund | Trustees & Officers (Unaudited)

March 31, 2026

INDEPENDENT TRUSTEES
Name (Age) |  | Position Held (Length of Time Served) |  | Principal Occupation (s) During Past 5 Years |  | Number of Portfolios in Fund Complex (1) Overseen by Trustee |  | Other Directorships Held During Past 5 Years
|  |  |  | National Committee. Former Vice Chairman of the Board of Overseers US Naval Academy. On the board of numerous charitable organizations. |  |  |  |
Walter H. Wilkinson, Jr. (80) |  | Trustee (since 2024) |  | Founder and former General Partner of Kitty Hawk Capital, a venture capital firm, from its founding in 1980 through 2016. |  | 3 |  | Gladstone Capital; Gladstone Investment; Gladstone Commercial; Gladstone Land; Gladstone Acquisition

(1) | The Fund Complex includes Gladstone Capital and Gladstone Investment, each a business development company advised by the Adviser.

(2) | Mr. Gladstone and Ms. Novara are interested persons of the Fund, within the meaning of Section 2(a)(19) of the 1940 Act, due to their positions as officers of the Fund and of the Adviser and their employment by the Adviser.

OFFICERS
Name (Age) |  | Position Held with the Fund |  | Year Appointed |  | Principal Occupation(s) During Past 5 Years
John Sateri (58) |  | President |  | Since Inception |  | Managing Director of the Adviser since 2007. Investment Committee member of the Adviser for Gladstone Land, Gladstone Investment, Gladstone Capital and Gladstone Commercial since 2021. President of the Fund since 2024. Chief Investment Officer of Gladstone Land, Gladstone Investment, Gladstone Capital and Gladstone Commercial since March 2026.
Michael Malesardi (65) |  | Chief Financial Officer and Treasurer |  | Since Inception |  | Chief Financial Officer and Treasurer of the Adviser since 2018. Chief Financial Officer and Treasurer of Gladstone Acquisition from January 2021 until October 2022. Chief Financial Officer and Treasurer of the Fund since 2024.
Michael LiCalsi (55) |  | Co-General Counsel and Co-Secretary |  | Since Inception |  | Chief Administrative Officer and Co-General Counsel and Co-Secretary for each of the Fund, Gladstone Capital,

Annual Report | March 31, 2026 |  | 37

Gladstone Alternative Income Fund | Trustees & Officers (Unaudited)

March 31, 2026

OFFICERS
Name (Age) |  | Position Held with the Fund |  | Year Appointed |  | Principal Occupation(s) During Past 5 Years
|  |  |  |  |  | Gladstone Investment, Gladstone Commercial and Gladstone Land since July 2025. General Counsel and Secretary of the Fund from inception prior to July 2025, and of Gladstone Capital, Gladstone Investment and Gladstone Commercial since 2009 and Gladstone Land since 2012. President of the Administrator since July 2013. Managing Principal and Chief Legal Officer of the Distributor and member of its board of managers since October 2010. General Counsel and Secretary of Gladstone Acquisition from January 2021 until October 2022.
Erich Hellmold (46) |  | Co-General Counsel and Co-Secretary |  | Since July 2025 |  | Co-General Counsel and Co-Secretary for Gladstone Land Corporation, Gladstone Investment Corporation, Gladstone Capital Corporation, Gladstone Commercial Corporation and Gladstone Alternative Income Fund since July 2025. Deputy General Counsel for Gladstone Land Corporation, Gladstone Investment Corporation, Gladstone Capital Corporation and Gladstone Commercial Corporation from July 2021 until July 2025 and Gladstone Alternative Income Fund from 2024 until July 2025. Assistant General Counsel for Gladstone Land Corporation, Gladstone Investment Corporation, Gladstone Capital Corporation and Gladstone Commercial Corporation from August 2017 until July 2021.

38 |  | www.gladstoneintervalfund.com

Gladstone Alternative Income Fund | Investor Data Privacy Notice

March 31, 2026 (Unaudited)
We are committed to maintaining the privacy of our shareholders and to safeguarding their
non-public
personal information. The following information is provided to help you understand what personal information we collect, how we protect that information and why, in certain cases, we may share information with select other parties.
Generally, we will not receive any nonpublic personal information about holders of the Shares, although certain of our shareholders’
non-public
information may become available to us. The
non-public
personal information that we may receive falls into the following categories:

| • |  | information we receive from shareholders. This includes shareholders’ communications to us concerning their investment;

| • |  | information about shareholders’ transactions and history with us; or

| • |  | other general information that we may obtain about shareholders, such as demographic and contact information (such as a shareholder’s address).

We do not disclose any
non-public
personal information about shareholders or former shareholders to anyone, except:

| • |  | to our affiliates (such as the Adviser and the Administrator) and their employees that have a legitimate business need for the information. The degree of access is based on the sensitivity of the information and on personnel need for the information to service a shareholder’s account or comply with legal requirements;

| • |  | to our service providers (such as our administrators, accountants, attorneys, custodians, transfer agent, underwriters and proxy solicitors) and their employees as is necessary to service shareholder accounts or otherwise provide the applicable service;

| • |  | to comply with court orders, subpoenas, lawful discovery requests, or other legal or regulatory requirements; or

| • |  | as allowed or required by applicable law or regulation.

When we share nonpublic shareholder personal information referred to above, the information is made available for limited business purposes and under controlled circumstances designed to protect our shareholders’ privacy. We do not permit use of shareholder information for any non-business or marketing purpose. We do not, and do not permit third parties to, rent, sell or trade personal information collected about you.
We maintain, and require our service providers to maintain, physical, electronic and procedural safeguards designed to protect the nonpublic personal information of our shareholders, to prevent unauthorized access or use and to dispose of such information when it is no longer required.
We may choose to modify our privacy policies at any time. Before we do so, we will notify Shareholders and provide a description of our privacy policy.
In the event of a corporate change in control resulting from, for example, a sale to, or merger with, another entity, or in the event of a sale of assets, we reserve the right to transfer your
non-public
personal information to the new party in control or the party acquiring assets.

Annual Report | March 31, 2026 |  | 39

(b) | Not applicable.

Item 2. Code of Ethics.

(a) | As of the end of the period covered by this report, the registrant has adopted a code of ethics that applies to the registrant’s principal executive officer, principal financial officer, principal accounting officer or controller, or persons performing similar functions, regardless of whether these individuals are employed by the registrant or a third party.

(c) | During the period covered by this report, the registrant’s Code of Ethics was amended to update certain language related to annual certification and reporting requirements and make certain clarifying edits to the conflicts of interest section.

(d) | During the period covered by the report, the registrant has not granted any express or implicit waivers from the provisions of the code of ethics.

(e) | Not applicable.

(f) | The registrant’s code of ethics referred to in Item 2(a) above is attached as Exhibit 19(a)(1) hereto.

Item 3. Audit Committee Financial Expert.

(a) | The registrant’s board of trustees has determined that the registrant has at least one audit committee financial expert serving on its audit committee. The registrant’s board of trustees has determined that Anthony W. Parker is an audit committee financial expert. Mr. Parker is “independent” as defined in paragraph (a)(2) of Item 3 to Form N-CSR.

Item 4. Principal Accountant Fees and Services.

(a) | Audit Fees

March 31, 2026 - $147,663

March 31, 2025 - $181,000

Audit fees represent the aggregate fees expected to be billed for the fiscal years ended March 31, 2026 and March 31, 2025 for professional services rendered by the principal accountant for the audit of the registrant’s annual financial statements or services that are normally provided by the accountant in connection with statutory and regulatory filings or engagements.

(b) | Audit-Related Fees

March 31, 2026 - $6,500

March 31, 2025 - $0

The fees paid for the fiscal year ended March 31, 2026 were related to the preparation of the post-effective amendment to the registrant’s Form N-2. The registrant was not billed for any fees for the fiscal year ended March 31, 2025 for assurance and related services that were reasonably related to the performance of the audit of the registrant’s financial statement and not otherwise included under “Audit Fees” above.

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(c) | Tax Fees

March 31, 2026 - $0

March 31, 2025 - $0

“Tax fees” shown above were for the preparation of Federal & State income tax returns, assistance with calculation of required income, capital gain and excise distributions and preparation of Federal excise tax returns for the fiscal years ended March 31, 2026 and March 31, 2025.

(d) | All Other Fees

March 31, 2026 - $0

March 31, 2025 - $0

The registrant was not billed any fees for products and services not otherwise included in items (a) – (c) shown above for the fiscal years ended March 31, 2026 and March 31, 2025.

(e) | (1)Audit Committee’s Pre-Approval Policies and Procedures

The Audit Committee is required to pre-approve the audit and non-audit services performed by PricewaterhouseCoopers LLC (the “Auditor”) in order to ensure that the provision of such services to the registrant does not impair the independence of the Auditor. Unless a type of service to be provided by the Auditor has received general pre-approval, it will require specific pre-approval by the Audit Committee. Any proposed services exceeding pre-approved fee levels will require specific pre-approval by the Audit Committee.

| (2) | Percentages of Services Approved by the Audit Committee pursuant to paragraph (c)(7)(i)(C) of Rule 2-01 of Regulation S-X.

|  | 2026 |  |  | 2025 |
Audit Related |  |  | — | % |  |  | — | %
Tax Fees |  |  | — | % |  |  | — | %
Other Fees |  |  | N/A |  |  |  | N/A |

(f) | During the audit of registrant’s financial statements for the most recent fiscal year, less than 50 percent of the hours expended on the principal accountant’s engagement were attributed to work performed by persons other than the principal accountant’s full-time, permanent employees.

(g) | The aggregate non-audit fees billed by the registrant’s accountant for services rendered to the registrant, and rendered to the Gladstone Management Corporation, the registrant’s investment adviser (the “Adviser”) (not including any sub-adviser whose role is primarily portfolio management and is subcontracted with or overseen by another investment adviser), and any entity controlling, controlled by, or under common control with the Adviser that provides ongoing services to the registrant:

March 31, 2026: $0

March 31, 2025: $0

(h) | The registrant’s audit committee has considered whether the provision of non-audit services to the Adviser (not including any sub-adviser whose role is primarily portfolio management and is subcontracted with or overseen by another investment adviser), and any entity controlling, controlled by, or under common control with the Adviser that provides ongoing services to the registrant, that were not pre-approved pursuant to paragraph (c)(7)(ii) of Rule 2-01 of Regulation S-X, is compatible with maintaining the principal accountant’s independence.

(i) | Not applicable.

(j) | Not applicable.

-3-

Item 5. Audit Committee of Listed Registrants.

Not applicable to registrant.

Item 6. Investments.

(a) | The schedule of investments is included as part of the Annual Report to Stockholders filed under Item 1(a) of this report.

(b) | Not applicable to registrant.

Item 7. Financial Statements and Financial Highlights for Open-End Management Investment Companies.

Not applicable to registrant.

Item 8. Changes in and Disagreements with Accountants for Open-End Management Investment Companies.

Not applicable to registrant.

Item 9. Proxy Disclosures for Open-End Management Investment Companies.

Not applicable to registrant.

Item 10. Remuneration Paid to Directors, Officers, and Others of Open-End Management Investment Companies.

Not applicable to registrant.

Item 11. Statement Regarding Basis for Approval of Investment Advisory Contract.

Not applicable to registrant.

Item 12. Disclosure of Proxy Voting Policies and Procedures for Closed-End Management Investment Companies.

See attached Appendix A for the Adviser’s proxy voting policy.

Item 13. Portfolio Managers of Closed-End Management Investment Companies.

(a)(1) | Identification of Portfolio Manager(s) or Management Team Members and Description of Role of Portfolio Manager(s) or Management Team Members

Portfolio Managers

The following are the portfolio managers as of the date of the filing of this report who are responsible for the day-to-day management of the registrant’s portfolio and the selection of its investments. Each individual listed below has served as a portfolio manager since our inception in 2024.

Name |  | Position(s) Held with Registrant |  | Principal Occupations Last 5 Years
David Gladstone |  | Trustee, Chairman, Chief Executive Officer |  | Founder, Chief Executive Officer of Gladstone Investment Corporation (“Gladstone Investment”) since its inception in 2005 until March 2026, of Gladstone Capital Corporation (“Gladstone Capital”) since its inception in 2001 until March 2026, of Gladstone Commercial Corporation (“Gladstone

-4-

|  |  |  | Commercial”) since its inception in 2003 until March 2026, and of Gladstone Land Corporation (“Gladstone Land”) since its inception in 1997 until March 2026. Chairman of the Board of Gladstone Investment since its inception in 2005, of Gladstone Capital since its inception in 2001, of Gladstone Commercial since its inception in 2003, of Gladstone Land since its inception in 1997. Founder, Chief Executive Officer and Chairman of the Board of the registrant since 2024. Founder, Chief Executive Officer and Chairman of the Board of our Adviser. Since 2010, Mr. Gladstone also serves on the board of managers of Gladstone Securities, LLC (the “Distributor”). Chief Executive Officer, President, Chief Investment Officer. Chief Executive Officer, President, Chief Investment Officer and Director of Gladstone Acquisition Corporation from January 2021 until October 2022.
John Sateri |  | President |  | Managing Director of the Adviser since 2007 and President of the registrant since its inception in 2024. Investment Committee member of the Adviser for Gladstone Land, Gladstone Investment, Gladstone Capital and Gladstone Commercial since 2021 and of the registrant since 2024. Chief Investment Officer of Gladstone Land, Gladstone Investment, Gladstone Capital and Gladstone Commercial since March 2026.
Laura Gladstone |  | Portfolio Manager |  | Managing Director of the Adviser since 2001. Investment Committee member of the Adviser for Gladstone Land, Gladstone Investment, Gladstone Capital and Gladstone Commercial since 2021 and of the registrant since 2024.

(a)(2) Other Accounts Managed by Portfolio Manager(s) or Management Team Member and Potential Conflicts of Interest

Other Accounts Managed by Portfolio Manager(s) or management Team

-5-

As of March 31, 2026:

Name of Portfolio Manager or Team Member |  | Type of Accounts |  | Total No. of Accounts Managed |  |  | Total Assets (millions) |  |  | No. of Accounts where Advisory Fee is Based on Performance |  |  | Total Assets in Accounts where Advisory Fee is Based on Performance (millions) |
David Gladstone |  | Registered Investment Companies: |  |  | — |  |  |  | — |  |  |  | — |  |  |  | — |
|  | Other Pooled Investment Vehicles: |  |  | 4 |  |  | $ | 4,682.6 |  |  |  | 4 |  |  | $ | 4,682.6 |
|  | Other Accounts: |  |  | — |  |  |  | — |  |  |  | — |  |  |  | — |
John Sateri |  | Registered Investment Companies: |  |  | — |  |  |  | — |  |  |  | — |  |  |  | — |
|  | Other Pooled Investment Vehicles: |  |  | 4 |  |  | $ | 4,682.6 |  |  |  | 4 |  |  | $ | 4,682.6 |
|  | Other Accounts: |  |  | — |  |  |  | — |  |  |  | — |  |  |  | — |
Laura Gladstone |  | Registered Investment Companies: |  |  | — |  |  |  | — |  |  |  | — |  |  |  | — |
|  | Other Pooled Investment Vehicles: |  |  | 4 |  |  | $ | 4,682.6 |  |  |  | 4 |  |  | $ | 4,682.6 |
|  | Other Accounts: |  |  | — |  |  |  | — |  |  |  | — |  |  |  | — |

Potential Conflicts of Interest

Our portfolio managers serve or may serve as portfolio managers, officers, directors, or principals of entities that operate in the same or a related line of business as we do or of investment funds managed by our affiliates. Accordingly, they may have obligations to investors in those entities, the fulfillment of which might not be in our or our shareholders’ best interests. For example, Mr. Gladstone, our chairman and chief executive officer, is the chairman of the board and chief executive officer of the Adviser, Gladstone Administration, LLC (the “Administrator”) and the Distributor, and is the chairman of the board of each of the publicly traded business development companies and real estate investment trusts managed by the Adviser (and is chief executive officer and president of one of the real estate investment trusts). In addition, Mr. Sateri, our president, is also Managing Director of the Adviser, and chief investment officer of the Adviser and each of the publicly traded business development companies and real estate investment trusts managed by the Adviser. Ms. Gladstone is also Managing Director of the Adviser. While portfolio managers and the officers and other employees of the Adviser devote as much time to the management of us as appropriate to enable the Adviser to perform its duties in accordance with the investment advisory agreement (the “Advisory Agreement”) between the registrant and the Adviser, the portfolio managers and other of the Adviser’s officers may have conflicts in allocating their time and services among us, on the one hand, and other investment vehicles managed by the Adviser, on the other hand. These activities could be viewed as creating a conflict of interest insofar as the time and effort of the portfolio managers and the officers and employees of the Adviser will not be devoted exclusively to our business but will instead be allocated between our business and the management of these other investment vehicles. Moreover, the Adviser currently manages, and may in the future establish or sponsor, investment vehicles which have overlapping investment objectives with ours and accordingly may invest in, whether principally or secondarily, asset classes we target. While the Adviser generally has broad authority to make investments on behalf of the investment vehicles that it advises, the Adviser has adopted investment allocation procedures to address these potential conflicts and intends to direct investment opportunities to us or the other funds that are managed by the Adviser with the investment strategy that most closely fits the investment opportunity. Nevertheless, the management of the Adviser may face conflicts in the allocation of investment opportunities to other entities it manages. As a result, it is possible that we may not be given the opportunity to participate in certain investments made by other funds managed by the Adviser.

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In certain circumstances, we may make investments in a portfolio company in which one of our affiliates has or will have an investment, subject to satisfaction of any regulatory restrictions and, where required, the prior approval of our Board of Trustees. As of October 1, 2024, the Board has approved the following types of transactions:

| • |  | Our affiliates, Gladstone Commercial and Gladstone Land, may, under certain circumstances, lease property to portfolio companies that we do not control. We may pursue such transactions only if (i) the portfolio company is not controlled by us or any of our affiliates, (ii) the portfolio company satisfies the tenant underwriting criteria of Gladstone Commercial or Gladstone Land, as applicable, and (iii) the transaction is approved by a majority of our independent trustees and a majority of the independent directors of Gladstone Commercial or Gladstone Land, as applicable. We expect that any such negotiations between Gladstone Commercial or Gladstone Land and our portfolio companies would result in lease terms consistent with the terms that the portfolio companies would be likely to receive were they not portfolio companies of ours.

| • |  | We may invest simultaneously with Gladstone Capital or Gladstone Investment in senior loans in the broadly syndicated market whereby neither we nor any affiliate has the ability to dictate the terms of the loans.

| • |  | Pursuant to an exemptive order (the “Co-Investment Order”) the SEC granted us,, we may co-invest, under certain circumstances, with certain of our affiliates, including Gladstone Capital, Gladstone Investment and any future business development company or closed-end management investment company that is advised (or sub-advised if it controls the fund) by the Adviser, or any combination of the foregoing subject to the conditions in the Co-Investment Order.

Certain of our portfolio managers or officers, who are also officers of the Adviser, may from time to time serve as directors of certain of our portfolio companies. If a portfolio manager or officer serves in such capacity for one of our portfolio companies, such individual will owe fiduciary duties to stockholders of the portfolio company, which duties may from time to time conflict with the interests of our shareholders.

In the course of our investing activities, we will pay management and incentive fees to the Adviser and will reimburse the Administrator for certain expenses it incurs. As a result, investors in our shares will invest on a “gross” basis and receive distributions on a “net” basis after expenses, resulting in, among other things, a lower rate of return than one might achieve through our investors themselves making direct investments. As a result of this arrangement, there may be times when the management team of the Adviser, including our portfolio managers, has interests that differ from those of our shareholders, giving rise to a conflict.

(a)(3) Compensation Structure of Portfolio Manager(s) or Management Team Members

As of March 31, 2026:

The Portfolio Managers receive compensation from the Adviser in the form of a base salary plus bonuses. Each Portfolio Manager’s base salary is determined by a review of salary surveys for persons with comparable experience who are serving in comparable capacities in the industry. Each Portfolio Manager’s base salary is set and reviewed yearly. Like all employees of the Adviser, a Portfolio Manager’s bonuses are tied to the post-tax performance of the Adviser and the entities that it advises. A Portfolio Manager’s bonuses increase or decrease when the Adviser’s income increases or decreases. The Adviser’s income, in turn, is directly tied to the management and incentive fees earned in managing its investment funds, including us. Pursuant to the Advisory Agreement, the Adviser receives a management fee and an incentive fee based on net investment income in excess of the hurdle rates as set out in the Advisory Agreement.

(a)(4) Disclosure of Securities Ownership

As of March 31, 2026, the Portfolio Managers ownership of the registrant was as follows:

Portfolio Managers |  | Dollar Range of Shares Owned
David Gladstone |  | over $1,000,000
John Sateri |  | $50,001-$100,000
Laura Gladstone |  | none

(b) | Not Applicable.

Item 14. Purchases of Equity Securities by Closed-End Management Investment Company and Affiliated Purchasers.

None.

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Item 15. Submission of Matters to a Vote of Security Holders.

None.

Item 16. Controls and Procedures.

(a) | Based on an evaluation of the registrant’s disclosure controls and procedures as of a date within 90 days prior to filing date of this Form N-CSR, the principal executive officer and principal financial officer of the registrant have concluded that the disclosure controls and procedures of the registrant are reasonably designed to ensure that the information required in filings on Form N-CSR is recorded, processed, summarized, and reported within the time periods specified in the Commission’s rules and forms, including that information required to be disclosed is accumulated and communicated to the registrant’s management, including the registrant’s principal executive officer and principal financial officer, as appropriate to allow timely decisions regarding required disclosure.

(b) | There were no changes in the registrant’s internal control over financial reporting that occurred during the period covered by this report that has materially affected, or are reasonably likely to materially affect, the registrant’s internal control over financial reporting.

Item 17. Disclosure of Securities Lending Activities for Closed-End Management Investment Companies.

(a) | Not applicable.

(b) | Not applicable.

Item 18. Recovery of Erroneously Awarded Compensation.

(a) | Not applicable.

(b) | Not applicable.

Item 19. Exhibits.

(a)(1) | Registrant’s Code of Ethics for Senior Financial Officers, which is the subject of the disclosure required by Item 2 of Form N-CSR, is attached hereto

(a)(2) | Not applicable.

(a)(3) | The certifications required by Rule 30a-2(a) of the Investment Company Act of 1940, as amended, are attached hereto

(a)(4) | Not applicable.

(a)(5) | Not applicable

(b) | The certifications for the registrant’s Principal Executive Officer and Principal Financial Officer, as required by Rule 30a-2(b) of the Investment Company Act of 1940, as amended, and Section 906 of the Sarbanes-Oxley Act of 2002 are attached hereto.

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SIGNATURES

Pursuant to the requirements of the Securities Exchange Act of 1934 and the Investment Company Act of 1940, the registrant has duly caused this report to be signed on its behalf by the undersigned, thereunto duly authorized.

Gladstone Alternative Income Fund

By: |  | /s/ David Gladstone
|  | David Gladstone
|  | Chief Executive Officer (principal executive officer)
Date: |  | June 3, 2026

Pursuant to the requirements of the Securities Exchange Act of 1934 and the Investment Company Act of 1940, this report has been signed below by the following persons on behalf of the registrant and in the capacities and on the dates indicated.

By: |  | /s/ David Gladstone
|  | David Gladstone
|  | Chief Executive Officer (principal executive officer)
Date: |  | June 3, 2026

By: |  | /s/ Michael Malesardi
|  | Michael Malesardi
|  | Chief Financial Officer (principal financial officer)
Date: |  | June 3, 2026

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